@article{Abouheif1998Limit-8352,
  year={1998},
  doi={10.1007/PL00006397},
  title={Limitations of metazoan 18S rRNA sequence data : implications for reconstructing a phylogeny of the animal kingdom and inferring the reality of the cambrian explosion},
  number={4},
  volume={47},
  issn={0022-2844},
  journal={Journal of Molecular Evolution},
  pages={394--405},
  author={Abouheif, Ehab and Zardoya, Rafael and Meyer, Axel}
}

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    <dc:contributor>Zardoya, Rafael</dc:contributor>
    <dc:creator>Abouheif, Ehab</dc:creator>
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    <dc:creator>Zardoya, Rafael</dc:creator>
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    <dc:contributor>Meyer, Axel</dc:contributor>
    <dcterms:issued>1998</dcterms:issued>
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    <dcterms:bibliographicCitation>First publ. in: Journal of Molecular Evolution 47 (1998), 4, pp. 394 405</dcterms:bibliographicCitation>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:54Z</dc:date>
    <dcterms:abstract xml:lang="eng">We document the phylogenetic behavior of the 18S rRNA molecule in 67 taxa from 28 metazoan phyla and assess the effects of among-site rate variation on reconstructing phylogenies of the animal kingdom. This empirical assessment was undertaken to clarify further the limits of resolution of the 18S rRNA gene as a phylogenetic marker and to address the question of whether 18S rRNA phylogenies can be used as a source of evidence to infer the reality of a Cambrian explosion. A notable degree of among-site rate variation exists between different regions of the 18S rRNA molecule, as well as within all classes of secondary structure. There is a significant negative correlation between inferred number of nucleotide substitutions and phylogenetic information, as well as with the degree of substitutional saturation within the molecule. Base compositional differences both within and between taxa exist and, in certain lineages, may be associated with long branches and phylogenetic position. Importantly, excluding sites with different degrees of nucleotide substitution significantly influences the topology and degree of resolution of maximum-parsimony phylogenies as well as neighbor-joining phylogenies (corrected and uncorrected for among-site rate variation) reconstructed at the metazoan scale. Together, these data indicate that the 18S rRNA molecule is an unsuitable candidate for reconstructing the evolutionary history of all metazoan phyla, and that the polytomies, i.e., unresolved nodes within 18S rRNA phylogenies, cannot be used as a single or reliable source of evidence to support the hypothesis of a Cambrian explosion.</dcterms:abstract>
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    <dc:creator>Meyer, Axel</dc:creator>
    <dc:contributor>Abouheif, Ehab</dc:contributor>
    <dcterms:title>Limitations of metazoan 18S rRNA sequence data : implications for reconstructing a phylogeny of the animal kingdom and inferring the reality of the cambrian explosion</dcterms:title>
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