KOPS - Das Institutionelle Repositorium der Universität Konstanz

Growth of Geobacter sulfurreducens with Acetate in Syntrophic Cooperation with Hydrogen-Oxidizing Anaerobic Partners

Growth of Geobacter sulfurreducens with Acetate in Syntrophic Cooperation with Hydrogen-Oxidizing Anaerobic Partners

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:b887ee229f4787333179f13fd051aa59

CORD-RUWISCH, Ralf, Derek R. LOVLEY, Bernhard SCHINK, 1998. Growth of Geobacter sulfurreducens with Acetate in Syntrophic Cooperation with Hydrogen-Oxidizing Anaerobic Partners. In: Applied and Environmental Microbiology. 64(6), pp. 2232-2236

@article{Cord-Ruwisch1998Growt-8349, title={Growth of Geobacter sulfurreducens with Acetate in Syntrophic Cooperation with Hydrogen-Oxidizing Anaerobic Partners}, year={1998}, number={6}, volume={64}, journal={Applied and Environmental Microbiology}, pages={2232--2236}, author={Cord-Ruwisch, Ralf and Lovley, Derek R. and Schink, Bernhard} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8349"> <dcterms:abstract xml:lang="eng">Pure cultures of Geobacter sulfurreducens and other Fe(III)-reducing bacteriaaccumulated hydrogen topartial pressures of 5 to 70 Pa with acetate, butyrate, benzoate, ethanol, lactate, or glucose as the electrondonor if electron release to an acceptor was limiting. G. sulfurreducens coupled acetateoxidation with electrontransfer to an anaerobic partner bacterium in the absence of ferric iron or other electron acceptors. Coculturesof G. sulfurreducens and Wolinella succinogenes with nitrate as the electron acceptor degraded acetate efficiently and grew with doubling times of 6 to 8 h. The hydrogen partial pressures in these acetate-degrading cocultures were considerably lower, in the range of 0.02 to 0.04 Pa. From these values and the concentrations of the other reactants, it was calculated that in this cooperation the free energy change available to G. sulfurreducens should be about 253 kJ per mol of acetate oxidized, assuming complete conversion of acetate to CO2 and H2. However, growth yields (18.5 g of dry mass per mol of acetate for the coculture, about 14 g for G. sulfurreducens) indicated considerably higher energy gains. These yield data, measurement of hydrogen production rates, and calculation of the diffusive hydrogen flux indicated that electron transfer in these cocultures may not proceed exclusively via interspecies hydrogen transfer but may also proceed through an alternative carrier system with higher redox potential, e.g., a c-type cytochrome that was found to be excreted by G. sulfurreducens into the culture fluid. Syntrophic acetate degradation was also possible with G. sulfurreducens and Desulfovibrio desulfuricans CSN but only with nitrate as electron acceptor. These cultures produced cell yields of 4.5 g of dry mass per mol of acetate, to which both partners contributed at about equal rates. These results demonstrate that some Fe(III)-reducing bacteria can oxidize organic compounds under Fe(III) limitation with the production of hydrogen, and they provide the first example of rapid acetate oxidation via interspecies electron transfer at moderate temperature.</dcterms:abstract> <dc:creator>Schink, Bernhard</dc:creator> <dc:creator>Lovley, Derek R.</dc:creator> <dcterms:title>Growth of Geobacter sulfurreducens with Acetate in Syntrophic Cooperation with Hydrogen-Oxidizing Anaerobic Partners</dcterms:title> <dc:contributor>Cord-Ruwisch, Ralf</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:bibliographicCitation>First publ. in: Applied and Environmental Microbiology 64 (1998), 6, pp. 2232-2236</dcterms:bibliographicCitation> <dc:language>eng</dc:language> <dc:rights>deposit-license</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:52Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:52Z</dc:date> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8349"/> <dc:contributor>Schink, Bernhard</dc:contributor> <dc:creator>Cord-Ruwisch, Ralf</dc:creator> <dc:format>application/pdf</dc:format> <dc:contributor>Lovley, Derek R.</dc:contributor> <dcterms:issued>1998</dcterms:issued> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Growth_of_Geobacter_sulfurreducens_with_Acetate_in_Syntrophic_1998.pdf 175

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto