Optical Study of Active Ion Transport in Lipid Vesicles Containing Reconstituted Na,K-ATPase
Optical Study of Active Ion Transport in Lipid Vesicles Containing Reconstituted Na,K-ATPase
Date
1985
Authors
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published in
The Journal of Membrane Biology ; 85 (1985), 1. - pp. 49-63. - ISSN 0022-2631. - eISSN 1432-1424
Abstract
A fluorescence method is described for the measurement of ATP-driven ion fluxes in lipid vesicles containing purified Na.K-ATPase. The membrane voltage of enzyme containing vesicles was measured by using a voltage-sensitive indocyanine dye. By addition of valinomycin the vesicle membrane is made selectively ermeable to K- so that the membrane voltage approaches the Nernst potential for K+. With constant external K+ concentration. the time course of internal K+ concentration can be continuously measured as change of the fluorescence signal after activation of the pump. The optical method has a higher time resolution than tracer-flux experiments and allows an accurate determination of initial flux rates. From the temperature dependence of active K+ transport its activation energy was determined to be 115 kJ/mol. ATP-stimulated electrogenic pumping can be measured as a fast fluorescence change when the membrane conductance is low (i.e., at low or zero valinomycin concentration). In accordance with expectation, the amplitude of the fast signal change increases with decreasing passive ion permeability of the vesicle membrane. The resolution of the charge movement is so high that a few pump turnovers can be easily detected.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Na,K-ATPase,reconstitution,potential sensitive dye,ion fluxes,transport kinetics,activation energy
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
APELL, Hans-Jürgen, Madeleine M. MARCUS, Beatrice M. ANNER, H. OETLIKER, Peter LÄUGER, 1985. Optical Study of Active Ion Transport in Lipid Vesicles Containing Reconstituted Na,K-ATPase. In: The Journal of Membrane Biology. 85(1), pp. 49-63. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/BF01872005BibTex
@article{Apell1985Optic-8334, year={1985}, doi={10.1007/BF01872005}, title={Optical Study of Active Ion Transport in Lipid Vesicles Containing Reconstituted Na,K-ATPase}, number={1}, volume={85}, issn={0022-2631}, journal={The Journal of Membrane Biology}, pages={49--63}, author={Apell, Hans-Jürgen and Marcus, Madeleine M. and Anner, Beatrice M. and Oetliker, H. and Läuger, Peter} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8334"> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8334/1/Optical_Study_of_charge.pdf"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8334/1/Optical_Study_of_charge.pdf"/> <dc:contributor>Anner, Beatrice M.</dc:contributor> <dcterms:title>Optical Study of Active Ion Transport in Lipid Vesicles Containing Reconstituted Na,K-ATPase</dcterms:title> <dc:language>eng</dc:language> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8334"/> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dc:contributor>Läuger, Peter</dc:contributor> <dc:format>application/pdf</dc:format> <dc:contributor>Apell, Hans-Jürgen</dc:contributor> <dc:contributor>Marcus, Madeleine M.</dc:contributor> <dc:creator>Anner, Beatrice M.</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:45Z</dcterms:available> <dc:creator>Apell, Hans-Jürgen</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:45Z</dc:date> <dc:contributor>Oetliker, H.</dc:contributor> <dcterms:abstract xml:lang="eng">A fluorescence method is described for the measurement of ATP-driven ion fluxes in lipid vesicles containing purified Na.K-ATPase. The membrane voltage of enzyme containing vesicles was measured by using a voltage-sensitive indocyanine dye. By addition of valinomycin the vesicle membrane is made selectively ermeable to K- so that the membrane voltage approaches the Nernst potential for K+. With constant external K+ concentration. the time course of internal K+ concentration can be continuously measured as change of the fluorescence signal after activation of the pump. The optical method has a higher time resolution than tracer-flux experiments and allows an accurate determination of initial flux rates. From the temperature dependence of active K+ transport its activation energy was determined to be 115 kJ/mol. ATP-stimulated electrogenic pumping can be measured as a fast fluorescence change when the membrane conductance is low (i.e., at low or zero valinomycin concentration). In accordance with expectation, the amplitude of the fast signal change increases with decreasing passive ion permeability of the vesicle membrane. The resolution of the charge movement is so high that a few pump turnovers can be easily detected.</dcterms:abstract> <dcterms:issued>1985</dcterms:issued> <dc:creator>Oetliker, H.</dc:creator> <dc:creator>Läuger, Peter</dc:creator> <dcterms:bibliographicCitation>First publ. in: The Journal of Membrane Biology 85 (1985), pp. 49-63</dcterms:bibliographicCitation> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:creator>Marcus, Madeleine M.</dc:creator> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No