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Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini

Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini

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SCHNELL, Sylvia, Bernhard SCHINK, 1991. Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini. In: Archives of Microbiology. 155(2), pp. 183-190. ISSN 0302-8933. eISSN 1432-072X

@article{Schnell1991Anaer-8330, title={Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini}, year={1991}, doi={10.1007/BF00248615}, number={2}, volume={155}, issn={0302-8933}, journal={Archives of Microbiology}, pages={183--190}, author={Schnell, Sylvia and Schink, Bernhard} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8330"> <dcterms:issued>1991</dcterms:issued> <dc:contributor>Schnell, Sylvia</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:language>eng</dc:language> <dcterms:title>Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini</dcterms:title> <dc:format>application/pdf</dc:format> <dc:contributor>Schink, Bernhard</dc:contributor> <dc:rights>deposit-license</dc:rights> <dcterms:abstract xml:lang="eng">The initial reactions involved in anaerobic aniline degradation by the sulfate-reducing Desulfobacterium anilini were studied. Experiments for substrate induction indicated the presence of a common pathway for aniline and 4-aminobenzoate, different from that for degradation of 2-aminobenzoate, 2-hydroxybenzoate, 4-hydroxybenzoate, or phenol. Degradation of aniline by dense cell suspensions depended on CO2 whereas 4-aminobenzoate degradation did not. If acetyl-CoA oxidation was inhibited by cyanide, benzoate accumulated during degradation of aniline or 4-aminobenzoate, indicating an initial carboxylation of aniline to 4-aminobenzoate, and further degradation via benzoate of both substrates. Extracts of aniline or 4-aminobenzoategrown cells activated 4-aminobenzoate to 4-aminobenzoyl-CoA in the presence of CoA, ATP and Mg 2+. 4-Aminobenzoyl-CoA-synthetase showed a Km for 4-aminobenzoate lower than 10 ~tM and an activity of 15.8 nmol - min- 1. mg- 1. 4-Aminobenzoyl-CoA was reductively deaminated to benzoyl-CoA by cell extracts in the presence of low-potential electron donors such as titanium citrate or cobalt sepulchrate (2.1 nmol - min -1 mg-1). Lower activities for the reductive deamination were measured with NADH or NADPH. Reductive deamination was also indicated by benzoate accumulation during 4-aminobenzoate degradation in cell suspensions under sulfate limitation. The results provide evidence that aniline is degraded via carboxylation to 4-aminobenzoate, which is activated to 4-aminobenzoyl-CoA and further metabolized by reductive deamination to benzoyl-CoA.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:43Z</dc:date> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8330"/> <dc:creator>Schink, Bernhard</dc:creator> <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 155 (1991), 2, pp. 183-190</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:43Z</dcterms:available> <dc:creator>Schnell, Sylvia</dc:creator> </rdf:Description> </rdf:RDF>

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