Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können am Montag, 6.2. und Dienstag, 7.2. keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted on Monday, Feb. 6 and Tuesday, Feb. 7.)
Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-50204 |
Author: | Ernst, Bernhard; Dietz, Lisa; Höger, Stefan J.; Dietrich, Daniel R. |
Year of publication: | 2005 |
Published in: | Environmental Toxicology ; 20 (2005), 4. - pp. 449-458. - ISSN 1520-4081. - eISSN 1522-7278 |
Pubmed ID: | 16007663 |
DOI (citable link): | https://dx.doi.org/10.1002/tox.20131 |
Summary: |
Cyanotoxins, particularly microcystins (MCs), have been shown to be a hazard to human health. MCs accumulate in aquatic organisms probably as a result of irreversible binding to liver protein phosphatases. The aim of this study was to describe the recovery of MC from fish liver using various detection methods, with MC-LR as the representative congener. These findings are discussed in conjunction with the current procedures and limit values used for human risk assessment. Following incubation of liver homogenates with various MC-LR concentrations, the homogenates were extracted by a water/methanol/butanol mixture via different treatments and subsequently analyzed via the colorimetric protein phosphatase inhibition assay (cPPA), HPLC, and anti-Adda ELISA. Detection via cPPA appeared to yield the highest recovery of MC-LR, although the presence of unspecific background may have resulted in overestimation of the true recovery. The recoveries determined via HPLC and anti-Adda ELISA were comparable to each other. The limits of detection were 0.01-2.4μg MC-LR/g liver tissue, depending on the method used. Maximum MC-LR recovery from samples incubated with 10 and 100μg MC-LR/g ranged between 44% and 101%. Recovery from samples incubated with 1μg MC-LR/g liver tissue was below 3%. Lower recovery is assumed to result from irreversible, covalent MC protein binding, as confirmed by Western blotting of liver homogenates with anti-Adda immunoprobing. The results demonstrate that further investigation of and improvement in routinely applied MC methods for fish tissue and/or food analyses are needed for a reliable risk assessment.
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Subject (DDC): | 570 Biosciences, Biology |
Keywords: | microcystin, recovery, fish, tissue, risk assessment, cyanobacteria, liver |
Link to License: | Attribution-NonCommercial-NoDerivs 2.0 Generic |
Bibliography of Konstanz: | Yes |
ERNST, Bernhard, Lisa DIETZ, Stefan J. HÖGER, Daniel R. DIETRICH, 2005. Recovery of MC-LR in fish liver tissue. In: Environmental Toxicology. 20(4), pp. 449-458. ISSN 1520-4081. eISSN 1522-7278. Available under: doi: 10.1002/tox.20131
@article{Ernst2005Recov-8323, title={Recovery of MC-LR in fish liver tissue}, year={2005}, doi={10.1002/tox.20131}, number={4}, volume={20}, issn={1520-4081}, journal={Environmental Toxicology}, pages={449--458}, author={Ernst, Bernhard and Dietz, Lisa and Höger, Stefan J. and Dietrich, Daniel R.} }
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Recovery_of_MC_LR_in_fish_liver_tissue.pdf | 1299 |