Recovery of MC-LR in fish liver tissue

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ERNST, Bernhard, Lisa DIETZ, Stefan J. HÖGER, Daniel R. DIETRICH, 2005. Recovery of MC-LR in fish liver tissue. In: Environmental Toxicology. 20(4), pp. 449-458. ISSN 1520-4081. eISSN 1522-7278

@article{Ernst2005Recov-8323, title={Recovery of MC-LR in fish liver tissue}, year={2005}, doi={10.1002/tox.20131}, number={4}, volume={20}, issn={1520-4081}, journal={Environmental Toxicology}, pages={449--458}, author={Ernst, Bernhard and Dietz, Lisa and Höger, Stefan J. and Dietrich, Daniel R.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8323"> <dc:creator>Ernst, Bernhard</dc:creator> <dcterms:title>Recovery of MC-LR in fish liver tissue</dcterms:title> <dc:format>application/pdf</dc:format> <dc:contributor>Dietrich, Daniel R.</dc:contributor> <dcterms:issued>2005</dcterms:issued> <dc:creator>Höger, Stefan J.</dc:creator> <dc:contributor>Höger, Stefan J.</dc:contributor> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:40Z</dc:date> <dc:creator>Dietz, Lisa</dc:creator> <dcterms:bibliographicCitation>First publ. in: Environmental Toxicology 20 (2005), 4, pp. 449-458</dcterms:bibliographicCitation> <dc:rights>deposit-license</dc:rights> <dc:creator>Dietrich, Daniel R.</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8323"/> <dcterms:abstract xml:lang="eng">Cyanotoxins, particularly microcystins (MCs), have been shown to be a hazard to human health. MCs accumulate in aquatic organisms probably as a result of irreversible binding to liver protein phosphatases. The aim of this study was to describe the recovery of MC from fish liver using various detection methods, with MC-LR as the representative congener. These findings are discussed in conjunction with the current procedures and limit values used for human risk assessment. Following incubation of liver homogenates with various MC-LR concentrations, the homogenates were extracted by a water/methanol/butanol mixture via different treatments and subsequently analyzed via the colorimetric protein phosphatase inhibition assay (cPPA), HPLC, and anti-Adda ELISA. Detection via cPPA appeared to yield the highest recovery of MC-LR, although the presence of unspecific background may have resulted in overestimation of the true recovery. The recoveries determined via HPLC and anti-Adda ELISA were comparable to each other. The limits of detection were 0.01-2.4μg MC-LR/g liver tissue, depending on the method used. Maximum MC-LR recovery from samples incubated with 10 and 100μg MC-LR/g ranged between 44% and 101%. Recovery from samples incubated with 1μg MC-LR/g liver tissue was below 3%. Lower recovery is assumed to result from irreversible, covalent MC protein binding, as confirmed by Western blotting of liver homogenates with anti-Adda immunoprobing. The results demonstrate that further investigation of and improvement in routinely applied MC methods for fish tissue and/or food analyses are needed for a reliable risk assessment.</dcterms:abstract> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:40Z</dcterms:available> <dc:contributor>Dietz, Lisa</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Ernst, Bernhard</dc:contributor> </rdf:Description> </rdf:RDF>

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