KOPS - The Institutional Repository of the University of Konstanz

Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss) how can environmental influences be detected?

Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können am Montag, 6.2. und Dienstag, 7.2. keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted on Monday, Feb. 6 and Tuesday, Feb. 7.)

Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss) how can environmental influences be detected?

Cite This

Files in this item

Checksum: MD5:52e149ef670485e5deedf0dad66791c8

KÖLLNER, Bernd, Birgit WASSERRAB, Günter KOTTERBA, Uwe FISCHER, 2002. Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss) how can environmental influences be detected?. In: Toxicology Letters. 131(1-2), pp. 83-95. ISSN 0378-4274. Available under: doi: 10.1016/S0378-4274(02)00044-9

@article{Kollner2002Evalu-8283, title={Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss) how can environmental influences be detected?}, year={2002}, doi={10.1016/S0378-4274(02)00044-9}, number={1-2}, volume={131}, issn={0378-4274}, journal={Toxicology Letters}, pages={83--95}, author={Köllner, Bernd and Wasserrab, Birgit and Kotterba, Günter and Fischer, Uwe} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8283"> <dc:contributor>Köllner, Bernd</dc:contributor> <dc:creator>Wasserrab, Birgit</dc:creator> <dc:contributor>Wasserrab, Birgit</dc:contributor> <dcterms:abstract xml:lang="eng">In fish, the first line of defense against infectious microorganisms is based on a broad range of nonspecific humoral and cellular immune mechanisms ( innate immunity ) which without prior specific activation can act in forming a more static barrier (Fish Shellfish Immunol. 10 (2000) 243; Dev. Comp. Immunol. 25 (2001) 827). This natural resistance is normally effective enough to protect fish from infectious diseases until specific immune responses are being induced (Fig. 1; Dev. Comp. Immunol. 25 (2001) 841). Healthy fish exhibit both nonspecific and specific immune responses depending directly on environmental temperature. Pollution of the natural aquatic environment with industrial or agricultural sewage is an important immunosuppressing factor resulting in higher susceptibility to infectious diseases. To date, the possible immunotoxicity of a substance is evaluated using quantification of humoral factors like lysozyme, complement, C-reactive protein or total immunoglobulins but less often using functional assays. Furthermore, most of the functional assays (phagocytosis, respiratory burst, proliferative response) are based on the measurement of the response of resting but not of specific activated immune cells. However, the physiological responses of the immune system to an infection are based on a complex, stepwise activation and proliferation, especially of the specific immune functions after first contact to the microorganisms. In this report we describe in vitro methods for the evaluation of cellular immune functions of different leukocyte populations after specific in vivo triggering of the immune system. Parameters to be evaluated are activation and proliferation of leukocyte populations, phagocytosis and respiratory burst, secretion of antigen-specific antibodies and specific cell-mediated cytotoxicity. Furthermore, challenge models with bacterial (Aeromonas salmonicida) and viral pathogens (Viral Haemorrhagic Septicemia Virus, VHSV) are presented.</dcterms:abstract> <dcterms:issued>2002</dcterms:issued> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Fischer, Uwe</dc:creator> <dcterms:bibliographicCitation>First publ. in: Toxicology Letters 131 (2002), 1-2, pp. 83-95</dcterms:bibliographicCitation> <dc:creator>Kotterba, Günter</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Fischer, Uwe</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:23Z</dcterms:available> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8283/1/Evaluation_of_immune_functions_of_rainbow_trout.pdf"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8283/1/Evaluation_of_immune_functions_of_rainbow_trout.pdf"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:23Z</dc:date> <dcterms:title>Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss) how can environmental influences be detected?</dcterms:title> <dc:creator>Köllner, Bernd</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8283"/> <dc:format>application/pdf</dc:format> <dc:contributor>Kotterba, Günter</dc:contributor> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Evaluation_of_immune_functions_of_rainbow_trout.pdf 960

This item appears in the following Collection(s)

Attribution-NonCommercial-NoDerivs 2.0 Generic Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.0 Generic

Search KOPS


My Account