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Peroxynitrite and Nitric Oxide Donors Induce Neuronal Apoptosis by Eliciting Autocrine Excitotoxicity

Peroxynitrite and Nitric Oxide Donors Induce Neuronal Apoptosis by Eliciting Autocrine Excitotoxicity

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Prüfsumme: MD5:98d19d0c8119152081ecc17881d5a89f

LEIST, Marcel, Eugenio FAVA, Cesare MONTECUCCO, Pierluigi NICOTERA, 1997. Peroxynitrite and Nitric Oxide Donors Induce Neuronal Apoptosis by Eliciting Autocrine Excitotoxicity. In: European Journal of Neuroscience. 9(7), pp. 1488-1498. ISSN 0953-816X. eISSN 1460-9568

@article{Leist1997Perox-8264, title={Peroxynitrite and Nitric Oxide Donors Induce Neuronal Apoptosis by Eliciting Autocrine Excitotoxicity}, year={1997}, doi={10.1111/j.1460-9568.1997.tb01503.x}, number={7}, volume={9}, issn={0953-816X}, journal={European Journal of Neuroscience}, pages={1488--1498}, author={Leist, Marcel and Fava, Eugenio and Montecucco, Cesare and Nicotera, Pierluigi} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8264"> <dc:rights>deposit-license</dc:rights> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:15Z</dcterms:available> <dc:format>application/pdf</dc:format> <dc:contributor>Leist, Marcel</dc:contributor> <dcterms:title>Peroxynitrite and Nitric Oxide Donors Induce Neuronal Apoptosis by Eliciting Autocrine Excitotoxicity</dcterms:title> <dcterms:bibliographicCitation>First publ. in: European Journal of Neuroscience ; 9 (1997), 7. - pp. 1488-1498</dcterms:bibliographicCitation> <dc:creator>Leist, Marcel</dc:creator> <dc:creator>Fava, Eugenio</dc:creator> <dcterms:abstract xml:lang="eng">Endogenous generation of nitric oxide and its congeners, including peroxynitrite (ONOO-), has been implicated in the mechanism of neuron loss in neurodegenerative diseases. Accordingly, nitric oxide donors and ONOO-can elicit both apoptosis and necrosis in neuron cultures. Here we show that nitric oxide donors and ONOO- are each able to trigger apoptosis of mouse cerebellar granule cells by an excitotoxic mechanism requiring exocytosis and NMDA receptor-mediated intracellular Ca2+ overload. This conclusion is supported by the following findings. Apoptosis was induced by various nitric oxide donors or by direct addition of ONOO- to differentiated cerebellar granule cell cultures that were sensitive to NMDA toxicity, but not in cerebellar granule cells that did not display NMDA-induced cell death (i.e. early days in culture) or in various glial cell populations. Donors of ONOO- or nitric oxide stimulated a sustained increase in intracellular Ca2+, which was prevented by inhibitors of NMDA receptors, such as MK-801 and 5-phospho-aminovaleric acid, or by dampening neuronal electrical activity with high concentrations of extracellular Mg2+. Moreover, these treatments and the exposure of cerebellar granule cells in nominally Ca2+-free media prevented apoptotic cell death. Both the intracellular Ca2+ increase and apoptosis elicited by ONOO- or the nitric oxide donors were prevented by blocking exocytosis with tetanus toxin or botulinum neurotoxin C.</dcterms:abstract> <dc:creator>Montecucco, Cesare</dc:creator> <dc:contributor>Montecucco, Cesare</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:15Z</dc:date> <dc:contributor>Nicotera, Pierluigi</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8264"/> <dc:creator>Nicotera, Pierluigi</dc:creator> <dcterms:issued>1997</dcterms:issued> <dc:contributor>Fava, Eugenio</dc:contributor> </rdf:Description> </rdf:RDF>

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