Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes

Thumbnail Image
Date
1997
Authors
Rosner, Bettina M.
Rainey, Frederick A.
Kroppenstedt, Reiner M.
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
ArXiv-ID
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published in
FEMS Microbiology Letters ; 148 (1997), 2. - pp. 175-180. - eISSN 0378-1097
Abstract
Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of R. opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(III) citrate. Expression of these enzymes was molybdenum-dependent. Acetylene hydratases in cell-free extracts of R. ruber and Gordona spp. did not require addition of reductants. No cross-reactivity could be found between cell-free extracts of any of these aerobic isolates and antibodies raised against the acetylene hydratase of the strictly anaerobic fermenting bacterium Pelobacter acetylenicus. These results show that acetylene hydratases are a biochemically heterogeneous group of enzymes.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Rhodococcus,Gordona,Pelobacter acetylenicus,acetylene hydratase,acetylene degradation
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690ROSNER, Bettina M., Frederick A. RAINEY, Reiner M. KROPPENSTEDT, Bernhard SCHINK, 1997. Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes. In: FEMS Microbiology Letters. 148(2), pp. 175-180. eISSN 0378-1097. Available under: doi: 10.1111/j.1574-6968.1997.tb10285.x
BibTex
@article{Rosner1997Acety-8258,
  year={1997},
  doi={10.1111/j.1574-6968.1997.tb10285.x},
  title={Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes},
  number={2},
  volume={148},
  journal={FEMS Microbiology Letters},
  pages={175--180},
  author={Rosner, Bettina M. and Rainey, Frederick A. and Kroppenstedt, Reiner M. and Schink, Bernhard}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8258">
    <dc:rights>terms-of-use</dc:rights>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8258/1/Acetylene_degradation_by_new_isolates_of_aerobic_bacteria_and_comparison_of_acetylene_hydratase_enzymes.pdf"/>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8258/1/Acetylene_degradation_by_new_isolates_of_aerobic_bacteria_and_comparison_of_acetylene_hydratase_enzymes.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Kroppenstedt, Reiner M.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8258"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:12Z</dc:date>
    <dc:contributor>Kroppenstedt, Reiner M.</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:12Z</dcterms:available>
    <dc:creator>Rosner, Bettina M.</dc:creator>
    <dcterms:abstract xml:lang="eng">Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of R. opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(III) citrate. Expression of these enzymes was molybdenum-dependent. Acetylene hydratases in cell-free extracts of R. ruber and Gordona spp. did not require addition of reductants. No cross-reactivity could be found between cell-free extracts of any of these aerobic isolates and antibodies raised against the acetylene hydratase of the strictly anaerobic fermenting bacterium Pelobacter acetylenicus. These results show that acetylene hydratases are a biochemically heterogeneous group of enzymes.</dcterms:abstract>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Rainey, Frederick A.</dc:contributor>
    <dcterms:title>Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes</dcterms:title>
    <dcterms:bibliographicCitation>First publ. in: FEMS Microbiology Letters 148 (2007), pp. 175-180</dcterms:bibliographicCitation>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dcterms:issued>1997</dcterms:issued>
    <dc:contributor>Rosner, Bettina M.</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <dc:creator>Rainey, Frederick A.</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No
Refereed