Polymorphic microsatellites for the lesser bulldog bat (Noctilio albiventris) cross-amplify with close and distant relatives

Abstract

We used the enriched genomic library to isolate 16 polymorphic microsatellite markers from the lesser bulldog bat (Noctilio albiventris). We analysed DNA from 226 individuals from two neighbouring populations to assess genetic polymorphism at these loci. Polymorphism varied from two to ten alleles per locus, with expected heterozygosity ranging from 0.309 to 0.766 (with the exception of one for which no P-value was calculated). Two loci, including the one mentioned above, departed from Hardy-Weinberg equilibrium and linkage disequilibrium was detected between one locus with two other loci after Bonferroni correction. All loci cross-amplified successfully with a member of at least one other bat family, several of them polymorphically. These loci will be used to study relatedness and parentage in a study on the social system of Noctilio albiventris.