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2-Aminobenzoyl-CoA monooxygenase/reductase, a novel type of flavoenzyme : studies on the stoichiometry and the course of the reaction

2-Aminobenzoyl-CoA monooxygenase/reductase, a novel type of flavoenzyme : studies on the stoichiometry and the course of the reaction

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BUDER, Reiner, Klaus ZIEGLER, Georg FUCHS, Bernd LANGKAU, Sandro GHISLA, 1989. 2-Aminobenzoyl-CoA monooxygenase/reductase, a novel type of flavoenzyme : studies on the stoichiometry and the course of the reaction. In: European Journal of Biochemistry. 185(3), pp. 637-643. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1989.tb15160.x

@article{Buder19892Amin-8209, title={2-Aminobenzoyl-CoA monooxygenase/reductase, a novel type of flavoenzyme : studies on the stoichiometry and the course of the reaction}, year={1989}, doi={10.1111/j.1432-1033.1989.tb15160.x}, number={3}, volume={185}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={637--643}, author={Buder, Reiner and Ziegler, Klaus and Fuchs, Georg and Langkau, Bernd and Ghisla, Sandro} }

Fuchs, Georg Ziegler, Klaus 2011-03-24T17:41:27Z eng Ghisla, Sandro application/pdf Ghisla, Sandro Fuchs, Georg Buder, Reiner The reaction catalyzed by 2-aminobenzoyl-coenzyme-A monooxygenase/reductase from a denitrifying Pseudomonas sp. has been investigated. 2-Aminobenzoyl-CoA and 2-amino[carboxy-14C]benzoyl-CoA were synthesized enzymatically using 2-aminobenzoyl-CoA synthetase from the same organism. The product was purified by chromatography and characterized by ultraviolet/visible and 1H-NMR spectroscopy. The conversion of 2-aminobenzoyl-CoA catalyzed by the monooxygenase/reductase requires NADH and oxygen, and yields at least two different products depending on the relative concentration of NADH. At [NADH]< Km (40 μM), i.e. [NADH]/[2-aminobenzoyl-CoA] ~ 0.02 0.05, the main product is probably a hydroxylated derivative of 2-aminobenzoyl-CoA, which is characterized by an absorbance maximum around 375 nm. When [NADH]/[2-aminobenzoyl-CoA] ~ 2 5, the predominant product is a non-aromatic coenzyme A thioester (λmax ~ 320 nm). The stoichiometry in this case is 2.1 2.4 mol NADH oxidized (mol oxygen consumed)−1 (mol 2-aminobenzoyl-CoA metabolized)−1. The product is extremely unstable in the acidic pH range and undergoes decarboxylation in a few minutes at pH < 5. Some degree of stabilisation is obtained upon reduction with sodium borohydride, probably resulting in a further reduced non-aromatic coenzyme-A thioester. Langkau, Bernd Buder, Reiner 2-Aminobenzoyl-CoA monooxygenase/reductase, a novel type of flavoenzyme : studies on the stoichiometry and the course of the reaction deposit-license Langkau, Bernd Ziegler, Klaus 1989 2011-03-24T17:41:27Z First publ. in: European Journal of Biochemistry 185 (1989), 3, pp. 637-643

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