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Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease

Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease

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MASILAMANI, Madhan, 2002. Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease [Dissertation]. Konstanz: University of Konstanz

@phdthesis{Masilamani2002Immun-8179, title={Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease}, year={2002}, author={Masilamani, Madhan}, address={Konstanz}, school={Universität Konstanz} }

Masilamani, Madhan Masilamani, Madhan Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) : Serum Soluble CD21 in Health and Disease application/pdf Immunologische Untersuchung des humanen Komplementrezeptors Typ II (CR2/CD21). Lösliches CD21 in Gesundheit und Krankheit In this study, the expression and regulation of membrane associated human complement receptor type-II (CR2/CD21) and its soluble form were investigated.<br />The expression of CD21 was analysed in normal and activated T cells. PCR analyses and immunofluorescence/confocal microscopy of peripheral blood T cells and of activated T cells showed reduction in CD21 mRNA and protein expression upon activation.<br />CD21 is released as a soluble form from the cell surface by proteolytic activity known as Shedding. Soluble CD21 was isolated and purified from human plasma to homogeneity applying affinity chromatography and density gradient centrifugation. Soluble CD21 was found to be a single 126 kDa molecular species. By determining the sedimentation coefficient of sCD21, the hydrodynamic properties such as diffusion coefficient and the frictional coefficient were calculated. These values show that the sCD21 isolated from human plasma is an elongated rod shaped molecule. A specific ELISA was developed using purified sCD21 as a standard for the determination of sCD21 concentration in human sera. Soluble CD21 levels were estimated in serum samples from 235 healthy donors, 209 RA patients, 17 patients with arthritis related disorders, 41 CVID patients, 10 healthy pregnant women and 10 neonates. The normal values of plasma sCD21 in healthy individuals of age between 20-40 years range from 100-500 ng/ml (median 292 ng/ml) with a tendency to decrease with age but did not differ with gender. sCD21 levels were significantly reduced in rheumatoid arthritis (P 2011-03-24T17:41:11Z eng 2011-03-24T17:41:11Z deposit-license 2002

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