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Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum

Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum

Type of Publication: Journal article
URI (citable link): http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-26451
Author: Müller, Jochen A.; Schink, Bernhard
Year of publication: 2000
Published in: Archives of Microbiology ; 173 (2000), 4. - pp. 288-295. - ISSN 0302-8933. - eISSN 1432-072X
DOI (citable link): https://dx.doi.org/10.1007/s002030000148
Summary:
The anaerobic bacterium Sporotomaculum hydroxybenzoicum ferments 3-hydroxybenzoate to acetate, butyrate, and CO2. 3-Hydroxybenzoate was activated to 3-hydroxybenzoyl-CoA in a CoA-transferase reaction with acetyl-CoA or butyryl-CoA as CoA donors. 3-Hydroxybenzoyl-CoA was reductively dehydroxylated, forming benzoyl-CoA. This reaction was measured in cell-free extracts with cob(I)alamin as low-potential electron donor. No evidence was obtained that cob(I)alamin is the physiological electron donor; however, inhibitor studies indicated involvement of a strong nucleophile in the reaction. Benzoate was degraded by dense cell suspensions without a lag phase until an in situ DG¢ value
Subject (DDC): 570 Biosciences, Biology
Keywords: Anaerobic degradation, Aromatic compounds, 3-Hydroxybenzoate, Benzoyl-CoA pathway, Reductive dehydroxylation, Fermenting bacteria
Link to License: Attribution-NonCommercial-NoDerivs 2.0 Generic

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MÜLLER, Jochen A., Bernhard SCHINK, 2000. Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum. In: Archives of Microbiology. 173(4), pp. 288-295. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s002030000148

@article{Muller2000Initi-8173, title={Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum}, year={2000}, doi={10.1007/s002030000148}, number={4}, volume={173}, issn={0302-8933}, journal={Archives of Microbiology}, pages={288--295}, author={Müller, Jochen A. and Schink, Bernhard} }

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