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A conserved motif is prerequisite for the interaction of NAC with ribosomal protein L23 and nascent chains

A conserved motif is prerequisite for the interaction of NAC with ribosomal protein L23 and nascent chains

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WEGRZYN, Renee D., Diana HOFMANN, Frieder MERZ, Rainer NIKOLAY, Thomas RAUCH, Christian GRAF, Elke DEUERLING, 2006. A conserved motif is prerequisite for the interaction of NAC with ribosomal protein L23 and nascent chains. In: Journal of Biological Chemistry. 281(5), pp. 2847-2857. ISSN 0021-9258. eISSN 1083-351X

@article{Wegrzyn2006conse-8150, title={A conserved motif is prerequisite for the interaction of NAC with ribosomal protein L23 and nascent chains}, year={2006}, doi={10.1074/jbc.M511420200}, number={5}, volume={281}, issn={0021-9258}, journal={Journal of Biological Chemistry}, pages={2847--2857}, author={Wegrzyn, Renee D. and Hofmann, Diana and Merz, Frieder and Nikolay, Rainer and Rauch, Thomas and Graf, Christian and Deuerling, Elke} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8150"> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8150"/> <dc:contributor>Wegrzyn, Renee D.</dc:contributor> <dcterms:issued>2006</dcterms:issued> <dcterms:title>A conserved motif is prerequisite for the interaction of NAC with ribosomal protein L23 and nascent chains</dcterms:title> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:41:00Z</dc:date> <dc:contributor>Hofmann, Diana</dc:contributor> <dc:creator>Nikolay, Rainer</dc:creator> <dc:creator>Hofmann, Diana</dc:creator> <dc:format>application/pdf</dc:format> <dc:creator>Wegrzyn, Renee D.</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:rights>deposit-license</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:41:00Z</dcterms:available> <dc:contributor>Deuerling, Elke</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Graf, Christian</dc:creator> <dc:contributor>Graf, Christian</dc:contributor> <dc:contributor>Rauch, Thomas</dc:contributor> <dcterms:abstract xml:lang="eng">In eukaryotes, newly synthesized proteins interact co-translationally with a multitude of different ribosome-bound factors and chaperones including the conserved heterodimeric nascent polypeptide-associated complex (NAC) and a Hsp40/70-based chaperone system. These factors are thought to play an important role in protein folding and targeting, yet their specific ribosomal localizations, which are prerequisite for their functions, remain elusive. This study describes the ribosomal localization of NAC and the molecular details by which NAC is able to contact the ribosome and gain access to nascent polypeptides. We identified a conserved RRK(X)nKK ribosome binding motif within the β-subunit of NAC that is essential for the entire NAC complex to attach to ribosomes and allow for its interaction with nascent polypeptide chains. The motif localizes within a potential loop region between two predicted β-helices in the N terminus of βNAC. This N-terminal βNAC ribosome-binding domain was completely portable and sufficient to target an otherwise cytosolic protein to the ribosome. NAC modified with a UV-activatable cross-linker within its ribosome binding motif specifically cross-linked to L23 ribosomal protein family members at the exit site of the ribosome, providing the first evidence of NAC-L23 interaction in the context of the ribosome. Mutations of L23 reduced NAC ribosome binding in vivo and in vitro, whereas other eukaryotic ribosome-associated factors such as the Hsp70/40 chaperones Ssb or Zuotin were unaffected. We conclude that NAC employs a conserved ribosome binding domain to position itself on the L23 ribosomal protein adjacent to the nascent polypeptide exit site.</dcterms:abstract> <dc:creator>Rauch, Thomas</dc:creator> <dc:contributor>Merz, Frieder</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Journal of Biological Chemistry 281 (2006), 5, pp. 2847 2857</dcterms:bibliographicCitation> <dc:contributor>Nikolay, Rainer</dc:contributor> <dc:creator>Merz, Frieder</dc:creator> <dc:creator>Deuerling, Elke</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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