Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-81796 |
Author: | Bonfoco, Emanuela; Leist, Marcel; Zhivotovsky, Boris; Orrenius, Sten; Lipton, Stuart A.; Nicotera, Pieriuigi |
Year of publication: | 1996 |
Published in: | Journal of Neurochemistry ; 67 (1996), 6. - pp. 2484-2493. - eISSN 1471-4159 |
DOI (citable link): | https://dx.doi.org/10.1046/j.1471-4159.1996.67062484.x |
Summary: |
We have recently demonstrated that nitric oxide (NO) donors can trigger either apoptosis or necrosis of neurons as a function of the intensity of the exposure. Here, we show that the apoptosis induced by the NO donors S-nitrosocysteine (SNOC) or S-nitroso-N-acetylpenicillamine (SNAP) in cultured cerebellar granule cells (CGCs) depends on NMDA receptor (NMDA-R) activation leading to intracellular Ca'+ overload. Early dissolution of actin filaments followed by breakdown of microtubUles and nuclear lam ins preceded the appearance of typical apoptotic features. NO donors induced tyrosine nitration in neurons, in a small population of contaminating astrocytes, and in cultures of cerebellar astroglial cells. However, astrocytes neither displayed cytoskeletal alterations nor underwent apoptosis. Competitive and uncompetitive NMDA receptor antagonists, such as Daminophosphonovaleric acid and MK-801, did not influence tyrosine nitration but prevented the accumulation of intracellular Ca", cytoskeletal breakdown, and apoptosis induced by either SNOC or SNAP in CGCs. Taken together, these data strongly suggest that Ca" influx through NMDA-R-gated ion channels is a critical event in CGC apoptosis induced by NO donors.
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Subject (DDC): | 540 Chemistry |
Keywords: | Apoptosis, Calcium, Cytoskeleton, Neurodegeneration, Nitric oxide |
Link to License: | In Copyright |
BONFOCO, Emanuela, Marcel LEIST, Boris ZHIVOTOVSKY, Sten ORRENIUS, Stuart A. LIPTON, Pieriuigi NICOTERA, 1996. Cytoskeletal Breakdown and Apoptosis Elicited by NO Donors in Cerebellar Granule Cells Require NMDA Receptor Activation. In: Journal of Neurochemistry. 67(6), pp. 2484-2493. eISSN 1471-4159. Available under: doi: 10.1046/j.1471-4159.1996.67062484.x
@article{Bonfoco1996Cytos-8149, title={Cytoskeletal Breakdown and Apoptosis Elicited by NO Donors in Cerebellar Granule Cells Require NMDA Receptor Activation}, year={1996}, doi={10.1046/j.1471-4159.1996.67062484.x}, number={6}, volume={67}, journal={Journal of Neurochemistry}, pages={2484--2493}, author={Bonfoco, Emanuela and Leist, Marcel and Zhivotovsky, Boris and Orrenius, Sten and Lipton, Stuart A. and Nicotera, Pieriuigi} }
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