Quantification of bacterial invasion into adherent cells by flow cytometry

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PILS, Stefan, Tim SCHMITTER, Florian NESKE, Christof R. HAUCK, 2006. Quantification of bacterial invasion into adherent cells by flow cytometry. In: Journal of Microbiological Methods. 65(2), pp. 301-310. ISSN 0167-7012. eISSN 1872-8359

@article{Pils2006Quant-8132, title={Quantification of bacterial invasion into adherent cells by flow cytometry}, year={2006}, number={2}, volume={65}, issn={0167-7012}, journal={Journal of Microbiological Methods}, pages={301--310}, author={Pils, Stefan and Schmitter, Tim and Neske, Florian and Hauck, Christof R.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8132"> <dc:creator>Neske, Florian</dc:creator> <dc:creator>Hauck, Christof R.</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8132"/> <dc:creator>Pils, Stefan</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:40:51Z</dc:date> <dc:contributor>Pils, Stefan</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Journal of Microbiological Methods 65 (2006), 2, pp. 301 310</dcterms:bibliographicCitation> <dc:language>eng</dc:language> <dc:creator>Schmitter, Tim</dc:creator> <dc:contributor>Hauck, Christof R.</dc:contributor> <dcterms:abstract xml:lang="deu">Quantification of invasive, intracellular bacteria is critical in many areas of cellular microbiology and immunology. We describe a novel and fast approach to determine invasion of bacterial pathogens in adherent cell types such as epithelial cells or fibroblasts based on flow cytometry. Using the CEACAM-mediated uptake of Opa-expressing Neisseria gonorrhoeae as a well-characterized model of bacterial invasion, we demonstrate that the flow cytometry-based method yields results comparable to a standard antibiotic protection assay. Furthermore, the quantification of intracellular bacteria by the novel approach is not biased by intracellular killing of the microbes and correctly discriminates between cell-associated extracellular and bona fide intracellular bacteria. As flow cytometry-based quantification is also applicable to other pathogen-host interactions such as the integrin-mediated intemalization of Staphylococcus aureus, this approach provides a fast and convenient alternative for the quantification of bacterial uptake and should be particularly useful in elucidating the molecular mechanisms of pathogen-triggered host cell invasion.</dcterms:abstract> <dc:contributor>Neske, Florian</dc:contributor> <dcterms:title>Quantification of bacterial invasion into adherent cells by flow cytometry</dcterms:title> <dcterms:issued>2006</dcterms:issued> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Schmitter, Tim</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:40:51Z</dcterms:available> <dc:rights>deposit-license</dc:rights> <dc:format>application/pdf</dc:format> </rdf:Description> </rdf:RDF>

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