Crystallographic analysis of AcrB
| Publikationstyp: | Zeitschriftenartikel |
| URI (zitierfähiger Link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-40351 |
| Autor/innen: | Pos, Klaas Martinus; Schiefner, André; Seeger, Markus A.; Diederichs, Kay |
| Erscheinungsjahr: | 2004 |
| Erschienen in: | FEBS Letters ; 564 (2004), 3. - S. 333-339. - ISSN 0014-5793 |
| DOI (zitierfähiger Link): | https://dx.doi.org/10.1016/S0014-5793(04)00272-8 |
| Zusammenfassung: |
A His-tagged derivative of the multidrug efflux pump AcrB could be crystallized in three different space groups (R3, R32 and P321). Experimental MAD-phasing maps from R32 AcrBHis crystals were obtained to a resolution of 3.5 Å. Datasets of native and substrate soaked AcrBHis crystals were collected at the Swiss Light Source X06SA beamline up to a resolution of 2.7 A+ and re¢nement of these data provided good quality electron density maps,which allowed us to complement the published AcrB structure (PDB code 1iwg). Introduction of amino acids 860-865 and 868 lacking in the 1iwg structure and deletion of a highly disordered region (amino acids 669-678) improved Rfree and average B factors in the 2.7 Å model. We could not identify signi¢cant densities indicating speci¢c antibiotic binding sites in the AcrB R32 space group datasets under the soaking conditions tested.
|
| Fachgebiet (DDC): | 570 Biowissenschaften, Biologie |
| Schlagwörter: | AcrB, Multidrug resistance, Membrane protein, X-ray crystallography |
| Link zur Lizenz: | Nutzungsbedingungen |
| Universitätsbibliographie: | Ja |
Zitieren
Dateien zu dieser Ressource
![[PDF]](/themes/Kops/images/mimes/pdf.png "PDF file"){kind=small}
POS, Klaas Martinus, André SCHIEFNER, Markus A. SEEGER, Kay DIEDERICHS, 2004. Crystallographic analysis of AcrB. In: FEBS Letters. 564(3), pp. 333-339. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(04)00272-8
@article{Pos2004Cryst-8129, title={Crystallographic analysis of AcrB}, year={2004}, doi={10.1016/S0014-5793(04)00272-8}, number={3}, volume={564}, issn={0014-5793}, journal={FEBS Letters}, pages={333--339}, author={Pos, Klaas Martinus and Schiefner, André and Seeger, Markus A. and Diederichs, Kay} }
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8129"> <dc:contributor>Schiefner, André</dc:contributor> <dc:language>eng</dc:language> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Seeger, Markus A.</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:40:50Z</dc:date> <dc:creator>Diederichs, Kay</dc:creator> <dc:creator>Pos, Klaas Martinus</dc:creator> <dcterms:bibliographicCitation>First publ. in: FEBS Letters 564 (2004), pp. 333-339</dcterms:bibliographicCitation> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8129/1/65_febs2004.pdf"/> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8129"/> <dc:creator>Seeger, Markus A.</dc:creator> <dc:contributor>Diederichs, Kay</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8129/1/65_febs2004.pdf"/> <dc:contributor>Pos, Klaas Martinus</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:abstract xml:lang="eng">A His-tagged derivative of the multidrug efflux pump AcrB could be crystallized in three different space groups (R3, R32 and P321). Experimental MAD-phasing maps from R32 AcrBHis crystals were obtained to a resolution of 3.5 Å. Datasets of native and substrate soaked AcrBHis crystals were collected at the Swiss Light Source X06SA beamline up to a resolution of 2.7 A+ and re¢nement of these data provided good quality electron density maps,which allowed us to complement the published AcrB structure (PDB code 1iwg). Introduction of amino acids 860-865 and 868 lacking in the 1iwg structure and deletion of a highly disordered region (amino acids 669-678) improved Rfree and average B factors in the 2.7 Å model. We could not identify signi¢cant densities indicating speci¢c antibiotic binding sites in the AcrB R32 space group datasets under the soaking conditions tested.</dcterms:abstract> <dc:rights>terms-of-use</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:40:50Z</dcterms:available> <dc:creator>Schiefner, André</dc:creator> <dcterms:title>Crystallographic analysis of AcrB</dcterms:title> <dc:format>application/pdf</dc:format> <dcterms:issued>2004</dcterms:issued> </rdf:Description> </rdf:RDF>
Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)
| 65_febs2004.pdf | 108 |
Das Dokument erscheint in:
Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: terms-of-use
KOPS Suche
Stöbern
Mein Benutzerkonto
