Crystallographic analysis of AcrB
| Type of Publication: | Journal article |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-40351 |
| Author: | Pos, Klaas Martinus; Schiefner, André; Seeger, Markus A.; Diederichs, Kay |
| Year of publication: | 2004 |
| Published in: | FEBS Letters ; 564 (2004), 3. - pp. 333-339. - ISSN 0014-5793 |
| DOI (citable link): | https://dx.doi.org/10.1016/S0014-5793(04)00272-8 |
| Summary: |
A His-tagged derivative of the multidrug efflux pump AcrB could be crystallized in three different space groups (R3, R32 and P321). Experimental MAD-phasing maps from R32 AcrBHis crystals were obtained to a resolution of 3.5 Å. Datasets of native and substrate soaked AcrBHis crystals were collected at the Swiss Light Source X06SA beamline up to a resolution of 2.7 A+ and re¢nement of these data provided good quality electron density maps,which allowed us to complement the published AcrB structure (PDB code 1iwg). Introduction of amino acids 860-865 and 868 lacking in the 1iwg structure and deletion of a highly disordered region (amino acids 669-678) improved Rfree and average B factors in the 2.7 Å model. We could not identify signi¢cant densities indicating speci¢c antibiotic binding sites in the AcrB R32 space group datasets under the soaking conditions tested.
|
| Subject (DDC): | 570 Biosciences, Biology |
| Keywords: | AcrB, Multidrug resistance, Membrane protein, X-ray crystallography |
| Link to License: | Attribution-NonCommercial-NoDerivs 2.0 Generic |
| Bibliography of Konstanz: | Yes |
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POS, Klaas Martinus, André SCHIEFNER, Markus A. SEEGER, Kay DIEDERICHS, 2004. Crystallographic analysis of AcrB. In: FEBS Letters. 564(3), pp. 333-339. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(04)00272-8
@article{Pos2004Cryst-8129, title={Crystallographic analysis of AcrB}, year={2004}, doi={10.1016/S0014-5793(04)00272-8}, number={3}, volume={564}, issn={0014-5793}, journal={FEBS Letters}, pages={333--339}, author={Pos, Klaas Martinus and Schiefner, André and Seeger, Markus A. and Diederichs, Kay} }
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