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Direct Comparison of Umbilical Cord Blood versus Bone Marrow Derived Endothelial Precursor Cells in Mediating Neovascularization in Response to Vascular Ischemia

Direct Comparison of Umbilical Cord Blood versus Bone Marrow Derived Endothelial Precursor Cells in Mediating Neovascularization in Response to Vascular Ischemia

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Prüfsumme: MD5:85dc75b6d6129c1b4d56cc6352a18198

FINNEY, Marcie R., Nicholas J. GRECO, Stephen E. HAYNESWORTH, Joseph M. MARTIN, David P. HEDRICK, Jimmy Z. SWAN, Daniel G. WINTER, Suzanne KADEREIT, Matthew E. JOSEPH, Pingfu FU, Vincent J. POMPILI, Mary J. LAUGHLIN, 2006. Direct Comparison of Umbilical Cord Blood versus Bone Marrow Derived Endothelial Precursor Cells in Mediating Neovascularization in Response to Vascular Ischemia. In: Biology of Blood and Marrow Transplantation. 12(5), pp. 585-593. ISSN 1083-8791. Available under: doi: 10.1016/j.bbmt.2005.12.037

@article{Finney2006Direc-8126, title={Direct Comparison of Umbilical Cord Blood versus Bone Marrow Derived Endothelial Precursor Cells in Mediating Neovascularization in Response to Vascular Ischemia}, year={2006}, doi={10.1016/j.bbmt.2005.12.037}, number={5}, volume={12}, issn={1083-8791}, journal={Biology of Blood and Marrow Transplantation}, pages={585--593}, author={Finney, Marcie R. and Greco, Nicholas J. and Haynesworth, Stephen E. and Martin, Joseph M. and Hedrick, David P. and Swan, Jimmy Z. and Winter, Daniel G. and Kadereit, Suzanne and Joseph, Matthew E. and Fu, Pingfu and Pompili, Vincent J. and Laughlin, Mary J.} }

Laughlin, Mary J. Swan, Jimmy Z. Hedrick, David P. Martin, Joseph M. 2011-03-24T17:40:49Z Pompili, Vincent J. Fu, Pingfu Swan, Jimmy Z. Martin, Joseph M. Joseph, Matthew E. Endothelial precursor cells (EPCs) cultured from adult bone marrow (BM) have been shown to mediate neovasculogenesis in murine models of vascular injury. We sought to directly compare umbilical cord blood (UCB)- and BM-derived EPC surface phenotypes and in vivo functional capacity. UCB and BM EPCs derived from mononuclear cells (MNC) were phenotyped by surface staining for expression of stromal (Stro-1, CXCR4, CD105, and CD73), endothelial (CD31, CD146, and vascular endothelial [VE]-cadherin), stem cell (CD34 and CD133), and monocyte (CD14) surface markers and analyzed by flow cytometry. The nonobese diabetic/severe combined immunodeficiency murine model of hind-limb ischemia was used to analyze the potential of MNCs and culturederived EPCs from UCB and BM to mediate neovasculogenesis. Histologic evaluation of the in vivo studies included capillary density as a measure of eovascularization. Surface CXCR4 expression was notably higher on UCB-derived EPCs (64.29% ± 7.41%) compared with BM (19.69% ± 5.49%; P = .021). Although the 2 sources of EPCs were comparable in expression of endothelial and monocyte markers, BM-derived EPCs contained higher proportions of cells expressing stromal cell markers (CD105 and CD73). Injection of UCB- or BM-derived EPCs resulted in significantly improved perfusion as measured by laser Doppler imaging at days 7 and 14 after femoral artery ligation in nonobese diabetic/severe combined immunodeficiency mice compared with controls (P < .05). Injection of uncultured MNCs from BM or UCB showed no significant difference from control mice (P = .119; P = .177). Tissue samples harvested from the lower calf muscle at day 28 demonstrated increased capillary densities in mice receiving BM- or UCB-derived EPCs. In conclusion, we found that UCB and BM-derived EPCs differ in CXCR4 expression and stromal surface markers but mediate equivalent neovasculogenesis in vivo as measured by Doppler flow and histologic analyses. Greco, Nicholas J. Kadereit, Suzanne First publ. in: Biology of Blood and Marrow Transplantation 12 (2006), 5, pp. 585-593 Joseph, Matthew E. Laughlin, Mary J. deposit-license eng application/pdf 2006 Haynesworth, Stephen E. Finney, Marcie R. Winter, Daniel G. Winter, Daniel G. Pompili, Vincent J. Greco, Nicholas J. Hedrick, David P. Finney, Marcie R. Fu, Pingfu Haynesworth, Stephen E. Kadereit, Suzanne 2011-03-24T17:40:49Z Direct Comparison of Umbilical Cord Blood versus Bone Marrow Derived Endothelial Precursor Cells in Mediating Neovascularization in Response to Vascular Ischemia

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