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Interactions between Diatoms and Bacteria from phototrophic Biofilms of the littoral Zone of Lake Constance

Interactions between Diatoms and Bacteria from phototrophic Biofilms of the littoral Zone of Lake Constance

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BRUCKNER, Christian G., 2008. Interactions between Diatoms and Bacteria from phototrophic Biofilms of the littoral Zone of Lake Constance

@phdthesis{Bruckner2008Inter-8122, title={Interactions between Diatoms and Bacteria from phototrophic Biofilms of the littoral Zone of Lake Constance}, year={2008}, author={Bruckner, Christian G.}, address={Konstanz}, school={Universität Konstanz} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8122"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:40:46Z</dcterms:available> <dcterms:title>Interactions between Diatoms and Bacteria from phototrophic Biofilms of the littoral Zone of Lake Constance</dcterms:title> <dc:creator>Bruckner, Christian G.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:40:46Z</dc:date> <dc:format>application/pdf</dc:format> <dc:contributor>Bruckner, Christian G.</dc:contributor> <dc:rights>deposit-license</dc:rights> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8122"/> <dcterms:alternative>Wechselwirkungen zwischen Kieselalgen und Bakterien aus phototrophen Biofilmen des Bodenseelitorals</dcterms:alternative> <dcterms:issued>2008</dcterms:issued> <dcterms:abstract xml:lang="eng">In this study we developed methods to purify diatoms from associated bacteria. Therefore most diatoms could be purified by short term harsh antibiotics treatment followed by single cell care. Diatoms that are difficult to purify could be made axenic via an intermediate defined co-culture with Escherichia coli. Phylogenetic analysis via 16S rRNA gene profiling of diatom associated bacteria indicated that Alphaproteobacteria and Bacteroidetes have adapted to the micro environment diatom biofilm. Defined co-cultures of diatoms and bacteria support this hypothesis by indicating that different bacteria utilize different fractions of secreted diatom carbohydrates. Bacteria were found to influence diatom growth strongly, either direct or by constitutively released soluble substances. We found concentrations of dissolved free amino acids (DFAA), analyzed by HPLC via ortho-phthaldialdehyde derivatization, to correlate with diatom growth when influenced by bacteria, and thus postulate that DFAA may be one class of such substances, regulating growth rate and growth density of diatoms. Further bacteria have a strong influence on the secretion of extracellular polymeric substances (EPS) by the diatoms. Investigating diatom carbohydrates by HPAE-PAD and spectrophotometric assays revealed that diatom carbohydrate secretion is influenced predominately quantitatively by bacteria. By quantifying diatom protein secretion and separating diatom proteins via SDS-PAGE we found diatom protein secretion to be influenced qualitatively and quantitatively by bacteria. The model organisms Phaeodactylum tricornutum and Escherichia coli were used to identify via Maldi-tof mass spectrometry peptide mass fingerprinting extracellular bacteria and diatom proteins that are induced during biofilm formation as an interaction of these organisms. These proteins could be related to different functions that are thought to play an important role during this interaction.</dcterms:abstract> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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