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Protein-Lipid Interactions with Fusobacterium nucleatum Major Outer Membrane Protein FomA : Spin-Label EPR and Polarized Infrared Spectroscopy

Protein-Lipid Interactions with Fusobacterium nucleatum Major Outer Membrane Protein FomA : Spin-Label EPR and Polarized Infrared Spectroscopy

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KLEINSCHMIDT, Jörg H., Derek MARSH, N. VIJAY, Veerappan ANBAZHAGAN, 2008. Protein-Lipid Interactions with Fusobacterium nucleatum Major Outer Membrane Protein FomA : Spin-Label EPR and Polarized Infrared Spectroscopy. In: Biochemistry. 47(37), pp. 8414-8423. ISSN 0006-2960. eISSN 1520-4995

@article{Kleinschmidt2008Prote-8118, title={Protein-Lipid Interactions with Fusobacterium nucleatum Major Outer Membrane Protein FomA : Spin-Label EPR and Polarized Infrared Spectroscopy}, year={2008}, doi={10.1021/bi800750s}, number={37}, volume={47}, issn={0006-2960}, journal={Biochemistry}, pages={8414--8423}, author={Kleinschmidt, Jörg H. and Marsh, Derek and Vijay, N. and Anbazhagan, Veerappan} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8118"> <dc:creator>Vijay, N.</dc:creator> <dcterms:title>Protein-Lipid Interactions with Fusobacterium nucleatum Major Outer Membrane Protein FomA : Spin-Label EPR and Polarized Infrared Spectroscopy</dcterms:title> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:40:45Z</dc:date> <dc:contributor>Vijay, N.</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Marsh, Derek</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:contributor>Anbazhagan, Veerappan</dc:contributor> <dc:creator>Anbazhagan, Veerappan</dc:creator> <dc:creator>Marsh, Derek</dc:creator> <dcterms:bibliographicCitation>First publ. in: Biochemistry ; 47 (2008), 37. - S. 8414 8423</dcterms:bibliographicCitation> <dcterms:issued>2008</dcterms:issued> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8118"/> <dc:format>application/pdf</dc:format> <dc:creator>Kleinschmidt, Jörg H.</dc:creator> <dc:contributor>Kleinschmidt, Jörg H.</dc:contributor> <dcterms:abstract xml:lang="eng">FomA, the major outer membrane protein of Fusobacterium nucleatum, was expressed and purified in Escherichia coli and reconstituted from detergent in bilayer membranes of phosphatidylcholines with chain lengths from C(12:0) to C(17:0). The conformation and orientation of membrane-incorporated FomA were determined from polarized, attenuated total reflection, infrared (IR) spectroscopy, and lipid-protein interactions with FomA were characterized by using electron paramagnetic resonance (EPR) spectroscopy of spin-labeled lipids. Approximately 190 residues of membranous FomA are estimated to be in a !-sheet configuration from IR band fitting, which is consistent with a 14-strand transmembrane !-barrel structure. IR dichroism of FomA indicates that the !-strands are tilted by !45° relative to the sheet/barrel axis and that the order parameter of the latter displays a discontinuity corresponding to hydrophobic matching with fluid C(13:0) lipid chains. The stoichiometry (Nb ) 23 lipids/monomer) of lipid-protein interaction from EPR demonstrates that FomA is not trimeric in membranes of diC(14:0) phosphatidylcholine and is consistent with a monomeric !-barrel of 14-16 strands. The pronounced selectivity of interaction found with anionic spin-labeled lipids places basic residues of the protein in the vicinity of the polar-apolar membrane interfaces, consistent with current topology models. Comparison with similar data from the 8- to 22-stranded E. coli outer membrane proteins, OmpA, OmpG, and FhuA, supports the above conclusions.</dcterms:abstract> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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