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The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift

The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift

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DEUERLING, Elke, Bettina PAESLACK, Wolfgang SCHUMANN, 1995. The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift. In: Journal of Bacteriology. 177(14), pp. 4105-4112

@article{Deuerling1995Bacil-8093, title={The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift}, year={1995}, number={14}, volume={177}, journal={Journal of Bacteriology}, pages={4105--4112}, author={Deuerling, Elke and Paeslack, Bettina and Schumann, Wolfgang} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8093"> <dc:rights>deposit-license</dc:rights> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:bibliographicCitation>First publ. in: Journal of Bacteriology 177 (1995), 14, pp. 4105-4112</dcterms:bibliographicCitation> <dc:creator>Schumann, Wolfgang</dc:creator> <dc:creator>Deuerling, Elke</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8093"/> <dc:contributor>Schumann, Wolfgang</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:53Z</dc:date> <dc:creator>Paeslack, Bettina</dc:creator> <dc:language>eng</dc:language> <dcterms:title>The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift</dcterms:title> <dc:format>application/pdf</dc:format> <dcterms:issued>1995</dcterms:issued> <dcterms:abstract xml:lang="eng">The ftsH gene of Bacillus subtilis has been identified as a salt-sensitive insertion mutation in strain UG1. Here, we show that UG1 has an insertion near the 3 end of ftsH. The salt sensitivity of this mutant was caused by reduction of ftsH mRNA levels by the synthesis of an artificial antisense RNA originating at a promoter located within the insertion and reading backwards into the ftsH gene. The salt-sensitive phenotype could be overcome by deleting the promoter from which the antisense RNA was transcribed. A physiological analysis of the isogenic wild-type strain in minimal medium revealed unimpaired growth at up to 1 M NaCl, and growth above 1.2 M NaCl was observed only after addition of the osmoprotectant proline or glycine betaine. In contrast, growth of strain UG1 was reduced at a salt concentration above 0.2 M, which could be rescued by the two compatible solutes already mentioned and also by trehalose. Primer extension revealed one potential transcription start site downstream of a putative vegetative promoter, which was activated after osmotic or temperature upshift. Northern (RNA blot) experiments led to the detection of a 2.1-kb transcript, suggesting that ftsH is monocistronic. A transcriptional fusion between ftsH and the gus reporter gene exhibited a twofold increase in b-glucuronidase activity after osmotic upshift. To further confirm the need for an enhanced level of FtsH protein after osmotic upshift, the promoter was replaced by the sucrose-inducible promoter PsacB. Whereas this mutant strain could grow in the absence of inducer in LB medium, it stopped growth immediately after addition of 1.1 M NaCl. We conclude that an increased amount of FtsH protein is essential for B. subtilis to cope with an increase in osmolarity or temperature.</dcterms:abstract> <dc:contributor>Deuerling, Elke</dc:contributor> <dc:contributor>Paeslack, Bettina</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:53Z</dcterms:available> </rdf:Description> </rdf:RDF>

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