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Map of the IncP1β Plasmid pTSA Encoding the Widespread Genes (tsa) for p-Toluenesulfonate Degradation in Comamonas testosteroni T-2

Map of the IncP1β Plasmid pTSA Encoding the Widespread Genes (tsa) for p-Toluenesulfonate Degradation in Comamonas testosteroni T-2

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TRALAU, Tewes, Alasdair M. COOK, Jürgen RUFF, 2001. Map of the IncP1β Plasmid pTSA Encoding the Widespread Genes (tsa) for p-Toluenesulfonate Degradation in Comamonas testosteroni T-2. In: Applied and Environmental Microbiology. 67(4), pp. 1508-1516. ISSN 0099-2240. Available under: doi: 10.1128/AEM.67.4.1508-1516.2001

@article{Tralau2001IncP1-8087, title={Map of the IncP1β Plasmid pTSA Encoding the Widespread Genes (tsa) for p-Toluenesulfonate Degradation in Comamonas testosteroni T-2}, year={2001}, doi={10.1128/AEM.67.4.1508-1516.2001}, number={4}, volume={67}, issn={0099-2240}, journal={Applied and Environmental Microbiology}, pages={1508--1516}, author={Tralau, Tewes and Cook, Alasdair M. and Ruff, Jürgen} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8087"> <dc:creator>Cook, Alasdair M.</dc:creator> <dc:format>application/pdf</dc:format> <dcterms:bibliographicCitation>First publ. in: Applied and Environmental Microbiology 67 (2001), 4, pp. 1508-1516</dcterms:bibliographicCitation> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:title>Map of the IncP1β Plasmid pTSA Encoding the Widespread Genes (tsa) for p-Toluenesulfonate Degradation in Comamonas testosteroni T-2</dcterms:title> <dc:contributor>Tralau, Tewes</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:50Z</dc:date> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:50Z</dcterms:available> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8087/1/Mapofthe_IncP1beta.pdf"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Ruff, Jürgen</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">The catabolic IncP1β plasmid pTSA from Comamonas testosteroni T-2 was mapped by subtractive analysis of restriction digests, by sequencing outwards from the tsa operon (toluenesulfonate degradation), and by generating overlapping, long-distance-PCR amplification products. The plasmid was estimated to comprise 72 +/- 4 kb. The tsa region was found to be a composite transposon flanked by two IS1071 elements. A cryptic tsa operon was also present in the tsa transposon. Those backbone genes and regions which we sequenced were in the same order as the corresponding genes in resistance plasmid R751, and identities of about 99% were observed. Enrichment cultures with samples from four continents were done to obtain organisms able to utilize p-toluenesulfonate as the sole source of carbon and energy for aerobic growth. Most (15) of the 16 cultures (13 of them isolates) were obtained from contaminated sites and were attributed to three metabolic groups, depending on their metabolism of p-toluenesulfonate. The largest group contained the tsa transposon, usually (six of seven isolates) with negligible differences in sequence from strain T-2.</dcterms:abstract> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8087/1/Mapofthe_IncP1beta.pdf"/> <dc:creator>Tralau, Tewes</dc:creator> <dcterms:rights rdf:resource="https://kops.uni-konstanz.de/page/termsofuse"/> <dcterms:issued>2001</dcterms:issued> <dc:creator>Ruff, Jürgen</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8087"/> </rdf:Description> </rdf:RDF>

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