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Required role of focal adhesion kinase (FAK) for integrin-stimulated cellmigration

Required role of focal adhesion kinase (FAK) for integrin-stimulated cellmigration


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SIEG, David J., Christof R. HAUCK, David D. SCHLAEPFER, 1999. Required role of focal adhesion kinase (FAK) for integrin-stimulated cellmigration. In: Journal of Cell Science. 112, pp. 2677-2691

@article{Sieg1999Requi-8080, title={Required role of focal adhesion kinase (FAK) for integrin-stimulated cellmigration}, year={1999}, volume={112}, journal={Journal of Cell Science}, pages={2677--2691}, author={Sieg, David J. and Hauck, Christof R. and Schlaepfer, David D.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8080"> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8080"/> <dc:contributor>Sieg, David J.</dc:contributor> <dcterms:title>Required role of focal adhesion kinase (FAK) for integrin-stimulated cellmigration</dcterms:title> <dc:creator>Hauck, Christof R.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:47Z</dcterms:available> <dcterms:issued>1999</dcterms:issued> <dc:contributor>Schlaepfer, David D.</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:47Z</dc:date> <dc:contributor>Hauck, Christof R.</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Journal of Cell Science 112 (1999), pp. 2677-2691</dcterms:bibliographicCitation> <dc:creator>Schlaepfer, David D.</dc:creator> <dc:language>eng</dc:language> <dc:rights>deposit-license</dc:rights> <dc:format>application/pdf</dc:format> <dc:creator>Sieg, David J.</dc:creator> <dcterms:abstract xml:lang="eng">FAK localizes to sites of transmembrane integrin receptor clustering and facilitates intracellular signaling events. FAK-null (FAK-) fibroblasts exhibit a rounded morphology, defects in cell migration, and an elevated number of cell-substratum contact sites. Here we show that stable re-expression of epitope-tagged FAK reversed the morphological defects of the FAK- cells through the dynamic regulation of actin structures and focal contact sites in fibronectin (FN) stimulated cells. FAK re-expressing fibroblasts (clones DA2 and DP3) exhibit a characteristic fibrillar shape and display indistinguishable FN receptorstimulated migration properties compared to normal fibroblasts. Expression of various FAK mutants in the FAK- cells showed that FAK kinase activity, the Tyr-397/SH2 domain binding site, and the first proline-rich SH3 binding region in the FAK C-terminal domain were individually needed to promote full FAK-mediated FAK-cell migration to FN whereas direct paxillin binding to FAK was not required. Expression of the FAK Phe-397 mutant did not promote FAK- cell migration and overexpression of p50csk in DA2 cells inhibited migration to FN suggesting that Src-family PTKs play important roles in FAK-mediated motility events. Expression of the FAK C-terminal domain, FRNK, promoted FAK dephosphorylation at Tyr-397 and potently blocked FAKmediated cell migration. This dominant-negative effect of FRNK was reversed by a point mutation (Leu-1034 to Ser) which prevented FRNK localization to focal contact sites. Our results show that FAK functions as a key regulator of fibronectin receptor stimulated cell migration events through the recruitment of both SH2 and SH3 domaincontaining signaling proteins to sites of integrin receptor clustering.</dcterms:abstract> </rdf:Description> </rdf:RDF>

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