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Complete anaerobic oxidation of hydroquinone by Desulfococcus sp. strain Hy5 : indications of hydroquinone carboxylation to gentisate

Complete anaerobic oxidation of hydroquinone by Desulfococcus sp. strain Hy5 : indications of hydroquinone carboxylation to gentisate

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GORNY, Norbert, Bernhard SCHINK, 1994. Complete anaerobic oxidation of hydroquinone by Desulfococcus sp. strain Hy5 : indications of hydroquinone carboxylation to gentisate. In: Archives of Microbiology. 162(1-2), pp. 131-135. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00264386

@article{Gorny1994Compl-8059, title={Complete anaerobic oxidation of hydroquinone by Desulfococcus sp. strain Hy5 : indications of hydroquinone carboxylation to gentisate}, year={1994}, doi={10.1007/BF00264386}, number={1-2}, volume={162}, issn={0302-8933}, journal={Archives of Microbiology}, pages={131--135}, author={Gorny, Norbert and Schink, Bernhard} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8059"> <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 162 (1994), 1-2, pp. 131-135</dcterms:bibliographicCitation> <dcterms:title>Complete anaerobic oxidation of hydroquinone by Desulfococcus sp. strain Hy5 : indications of hydroquinone carboxylation to gentisate</dcterms:title> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:36Z</dc:date> <dc:language>eng</dc:language> <dc:format>application/pdf</dc:format> <dcterms:issued>1994</dcterms:issued> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8059"/> <dc:contributor>Schink, Bernhard</dc:contributor> <dc:creator>Gorny, Norbert</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8059/1/Complete_anaerobic_oxidation_of_hydroquinone_1994.pdf"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8059/1/Complete_anaerobic_oxidation_of_hydroquinone_1994.pdf"/> <dc:creator>Schink, Bernhard</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Gorny, Norbert</dc:contributor> <dcterms:abstract xml:lang="eng">The sulfate-reducing strain Hy5 was able to grow with hydroquinone as sole source of carbon and energy. In experiments with dense cell suspensions, several indications were found that gentisate was the first intermediate in anaerobic degradation of hydroquinone: (1) degradation of hydroquinone was accelerated by addition of bicarbonate; (2) cell suspensions grown with hydroquinone oxidized gentisate at a rate similar to that of suspensions grown with gentisate, whereas the latter were not able to degrade hydroquinone in the presence of hloramphenicol;(3) in SDS-PAGE analysis of cell-free extracts of strain Hy5, two additional protein bands were found after growth with hydroquinone that were not detected in cells grown with gentisate, probably representing a hydroquinone carboxylating enzyme. A corresponding enzyme activity could not be detected. In cell-free extracts of hydroquinone-grown strain Hy5, the specific acyl-CoA ligase activity with gentisate as substrate was detected at 70 nmol mg -1 x min-L Gentisyl-CoA was enzymatically reduced to several unidentified nonaromatic products in the presence of dithionite-reduced methyl viologen.</dcterms:abstract> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:36Z</dcterms:available> <dc:rights>terms-of-use</dc:rights> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> </rdf:Description> </rdf:RDF>

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