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Enhanced Propionate Formation by Propionibacterium freudenreichii subsp. freudenreichii in a Three-Electrode Amperometric Culture System

Enhanced Propionate Formation by Propionibacterium freudenreichii subsp. freudenreichii in a Three-Electrode Amperometric Culture System

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Prüfsumme: MD5:045f81de8a69b02c062c32a5d9e80892

EMDE, Rainer, Bernhard SCHINK, 1990. Enhanced Propionate Formation by Propionibacterium freudenreichii subsp. freudenreichii in a Three-Electrode Amperometric Culture System. In: Applied and Environmental Microbiology. 56(9), pp. 2771-2776

@article{Emde1990Enhan-8045, title={Enhanced Propionate Formation by Propionibacterium freudenreichii subsp. freudenreichii in a Three-Electrode Amperometric Culture System}, year={1990}, number={9}, volume={56}, journal={Applied and Environmental Microbiology}, pages={2771--2776}, author={Emde, Rainer and Schink, Bernhard} }

2011-03-24T17:39:30Z Schink, Bernhard eng Schink, Bernhard application/pdf Enhanced Propionate Formation by Propionibacterium freudenreichii subsp. freudenreichii in a Three-Electrode Amperometric Culture System 2011-03-24T17:39:30Z Emde, Rainer 1990 Emde, Rainer First publ. in: Applied and Environmental Microbiology 56 (1990), 9, pp. 2771-2776 deposit-license In order to influence the fermentation pattern of Propionibacterium freudenreichii towards enhanced propionate formation, growth and product formation with glucose and lactate as energy sources were studied in a three-electrode poised-potential amperometric culture system. With anthraquinone 2,6-disulfonic acid (Eo' = -184 mV; poised electron potential = -224 mV) or cobalt sepulchrate (Eo' = -350 mV; -390 mV) as mediator and an activated platinum working electrode, reduction of bacterially oxidized mediator occurred fast enough to keep more than 50% of the respective mediator (in minimum 0.4 mM) in the reduced state, up to a current of 2 mA. With glucose as substrate, 90.0 or 97.3% propionate was formed during exponential growth in the presence of 0.5 mM anthraquinone 2,6-disulfonic acid or 0.4 mM cobalt sepulchrate, respectively.<br />Growth yields of 56.3 or 53.8 g of cell material per mol of substrate degraded were calculated, respectively, and the electrons were transferred quantitatively from the working electrode to the bacterial cells. With L-lactate, only 68.6 or 72.9% propionate was formed with the same mediators. The results are discussed with respect to energetics, electron transfer potentials, and potential application of the new technique in technical propionate production.

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