Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-26806 |
Author: | Emde, Rainer; Schink, Bernhard |
Year of publication: | 1990 |
Published in: | Applied and Environmental Microbiology ; 56 (1990), 9. - pp. 2771-2776 |
Summary: |
In order to influence the fermentation pattern of Propionibacterium freudenreichii towards enhanced propionate formation, growth and product formation with glucose and lactate as energy sources were studied in a three-electrode poised-potential amperometric culture system. With anthraquinone 2,6-disulfonic acid (Eo' = -184 mV; poised electron potential = -224 mV) or cobalt sepulchrate (Eo' = -350 mV; -390 mV) as mediator and an activated platinum working electrode, reduction of bacterially oxidized mediator occurred fast enough to keep more than 50% of the respective mediator (in minimum 0.4 mM) in the reduced state, up to a current of 2 mA. With glucose as substrate, 90.0 or 97.3% propionate was formed during exponential growth in the presence of 0.5 mM anthraquinone 2,6-disulfonic acid or 0.4 mM cobalt sepulchrate, respectively.
Growth yields of 56.3 or 53.8 g of cell material per mol of substrate degraded were calculated, respectively, and the electrons were transferred quantitatively from the working electrode to the bacterial cells. With L-lactate, only 68.6 or 72.9% propionate was formed with the same mediators. The results are discussed with respect to energetics, electron transfer potentials, and potential application of the new technique in technical propionate production. |
Subject (DDC): | 570 Biosciences, Biology |
Link to License: | Attribution-NonCommercial-NoDerivs 2.0 Generic |
EMDE, Rainer, Bernhard SCHINK, 1990. Enhanced Propionate Formation by Propionibacterium freudenreichii subsp. freudenreichii in a Three-Electrode Amperometric Culture System. In: Applied and Environmental Microbiology. 56(9), pp. 2771-2776
@article{Emde1990Enhan-8045, title={Enhanced Propionate Formation by Propionibacterium freudenreichii subsp. freudenreichii in a Three-Electrode Amperometric Culture System}, year={1990}, number={9}, volume={56}, journal={Applied and Environmental Microbiology}, pages={2771--2776}, author={Emde, Rainer and Schink, Bernhard} }
Enhanced_Propionate_Formation_by_Propionibacterium_1990.pdf | 374 |