Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können kommenden Montag und Dienstag keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted next Monday and Tuesday.)
Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-40099 |
Author: | Kissmehl, Roland; Treptau, Tilman; Kottwitz, Brunhilde; Plattner, Helmut |
Year of publication: | 1997 |
Published in: | Archives of Biochemistry and Biophysics ; 344 (1997), 2. - pp. 260-270. - ISSN 0003-9861 |
Pubmed ID: | 9264538 |
DOI (citable link): | https://dx.doi.org/10.1006/abbi.1997.0208 |
Summary: |
Using para-nitrophenyl phosphate (pNPP) as a substrate for enzymatic activity, we sought to identify CaN inParamecium.We isolated three different pNPP-phosphatases from the soluble fraction of Parameciumcells by anion-exchange and affinity column chromatographies. One, pNPP-phosphatasePeak I, is very similar to mammalian CaN. Divalent cation dependency, inhibition by calmodulin (CaM) antagonists (trifluoperazine, calmidazolium), and insensitivity to various phosphatase inhibitors (heparin, okadaic acid, sodium vanadate, etc.) show similarity to mammalian CaN rather than to any other Paramecium pNPP-hydrolyzing enzymes tested. Polyclonal antibodies against bovine brain CaN recognizing subunits A (61 or 58 kDa) and B (17 kDa) of brain CaN cross-reacted with a 63-kDa protein in fractions containing Peak I pNPP-phosphatase activity and coeluted calmodulin. Overlay assays using biotinylated brain calmodulin indicated Ca2+-dependent CaM-binding by the 63-kDa protein. A Ca2+-binding protein with the same electrophoretic mobility as CaN B (17 kDa) was also present, though in other fractions from DEAE cellulose chromatography. This finding strongly suggests that, in the absence of Ca2+, both subunits, A and B, were separated either before or during chromatographic processing. Our data support the existence of both subunits of a CaN-like phosphatase in Paramecium cells.
|
Subject (DDC): | 570 Biosciences, Biology |
Keywords: | calcineurin, calmodulin, Paramecium, para-nitrophenyl phosphate, protein phosphatase |
Link to License: | Attribution-NonCommercial-NoDerivs 2.0 Generic |
KISSMEHL, Roland, Tilman TREPTAU, Brunhilde KOTTWITZ, Helmut PLATTNER, 1997. Occurrence of a para-nitrophenyl phosphate-phosphatase with calcineurin-like characteristics in Paramecium tetraurelia. In: Archives of Biochemistry and Biophysics. 344(2), pp. 260-270. ISSN 0003-9861. Available under: doi: 10.1006/abbi.1997.0208
@article{Kissmehl1997Occur-8039, title={Occurrence of a para-nitrophenyl phosphate-phosphatase with calcineurin-like characteristics in Paramecium tetraurelia}, year={1997}, doi={10.1006/abbi.1997.0208}, number={2}, volume={344}, issn={0003-9861}, journal={Archives of Biochemistry and Biophysics}, pages={260--270}, author={Kissmehl, Roland and Treptau, Tilman and Kottwitz, Brunhilde and Plattner, Helmut} }
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8039"> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8039/1/Occurrence_of_a_para.pdf"/> <dcterms:issued>1997</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:27Z</dc:date> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dc:creator>Kottwitz, Brunhilde</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8039/1/Occurrence_of_a_para.pdf"/> <dc:contributor>Kissmehl, Roland</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8039"/> <dc:creator>Kissmehl, Roland</dc:creator> <dcterms:abstract xml:lang="deu">Using para-nitrophenyl phosphate (pNPP) as a substrate for enzymatic activity, we sought to identify CaN inParamecium.We isolated three different pNPP-phosphatases from the soluble fraction of Parameciumcells by anion-exchange and affinity column chromatographies. One, pNPP-phosphatasePeak I, is very similar to mammalian CaN. Divalent cation dependency, inhibition by calmodulin (CaM) antagonists (trifluoperazine, calmidazolium), and insensitivity to various phosphatase inhibitors (heparin, okadaic acid, sodium vanadate, etc.) show similarity to mammalian CaN rather than to any other Paramecium pNPP-hydrolyzing enzymes tested. Polyclonal antibodies against bovine brain CaN recognizing subunits A (61 or 58 kDa) and B (17 kDa) of brain CaN cross-reacted with a 63-kDa protein in fractions containing Peak I pNPP-phosphatase activity and coeluted calmodulin. Overlay assays using biotinylated brain calmodulin indicated Ca2+-dependent CaM-binding by the 63-kDa protein. A Ca2+-binding protein with the same electrophoretic mobility as CaN B (17 kDa) was also present, though in other fractions from DEAE cellulose chromatography. This finding strongly suggests that, in the absence of Ca2+, both subunits, A and B, were separated either before or during chromatographic processing. Our data support the existence of both subunits of a CaN-like phosphatase in Paramecium cells.</dcterms:abstract> <dcterms:title>Occurrence of a para-nitrophenyl phosphate-phosphatase with calcineurin-like characteristics in Paramecium tetraurelia</dcterms:title> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:format>application/pdf</dc:format> <dcterms:bibliographicCitation>First publ. in: Archives of Biochemistry and Biophysics 344 (1997), 2, pp. 260-270</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Plattner, Helmut</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:27Z</dcterms:available> <dc:contributor>Treptau, Tilman</dc:contributor> <dc:creator>Treptau, Tilman</dc:creator> <dc:contributor>Kottwitz, Brunhilde</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Plattner, Helmut</dc:contributor> </rdf:Description> </rdf:RDF>
Occurrence_of_a_para.pdf | 552 |