Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1

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PHILIPP, Bodo, Henrike ERDBRINK, Marc J.-F. SUTER, Bernhard SCHINK, 2006. Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1. In: Archives of Microbiology. 185(3), pp. 192-201. ISSN 0302-8933. eISSN 1432-072X

@article{Philipp2006Degra-8016, title={Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1}, year={2006}, doi={10.1007/s00203-006-0085-9}, number={3}, volume={185}, issn={0302-8933}, journal={Archives of Microbiology}, pages={192--201}, author={Philipp, Bodo and Erdbrink, Henrike and Suter, Marc J.-F. and Schink, Bernhard} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8016"> <dcterms:title>Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1</dcterms:title> <dc:creator>Erdbrink, Henrike</dc:creator> <dc:contributor>Erdbrink, Henrike</dc:contributor> <dc:contributor>Philipp, Bodo</dc:contributor> <dc:format>application/pdf</dc:format> <dc:contributor>Schink, Bernhard</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 185 (2006), 3, pp.192-201</dcterms:bibliographicCitation> <dcterms:issued>2006</dcterms:issued> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:creator>Philipp, Bodo</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:17Z</dcterms:available> <dc:creator>Suter, Marc J.-F.</dc:creator> <dc:creator>Schink, Bernhard</dc:creator> <dc:contributor>Suter, Marc J.-F.</dc:contributor> <dc:language>eng</dc:language> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8016"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:17Z</dc:date> <dcterms:abstract xml:lang="eng">A facultative anaerobic bacterium, Pseudomonas sp. strain Chol1, degrading cholate and other bile acids was isolated from soil. We investigated how strain Chol1 grew with cholate and whether growth was affected by the toxicity of this compound. Under anoxic conditions with nitrate as electron acceptor, strain Chol1 grew by transformation of cholate to 7,12-dihydroxy-1,4-androstadiene-3,17-dione (DHADD) as end product. Under oxic conditions, strain Chol1 grew by transformation of cholate to 3,7,12-trihydroxy-9,10-seco-1,3,5(10)-androstatriene-9,17-dione (THSATD), which accumulated in the culture supernatant before its further oxidation to CO2. Strain Chol1 converted DHADD into THSATD by an oxygenase-dependent reaction. Addition of cholate ( 10 mM) to cell suspensions of strain Chol1 caused a decrease of optical density and viable counts but aerobic growth with these toxic cholate concentrations was possible. Addition of CCCP or EDTA strongly increased the sensitivity of the cells to 10 mM cholate. EDTA also increased the sensitivity of the cells to DHADD and THSATD ( £ 1.7 mM). The toxicity of cholate and its degradation intermediates with a steroid structure indicates that strain Chol1 requires a strategy to minimize these toxic effects during growth with cholate. Apparently, the proton motive force and the outer membrane are necessary for protection against these toxic effects.</dcterms:abstract> <dc:rights>deposit-license</dc:rights> </rdf:Description> </rdf:RDF>

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