Use of chromosome microdissection in fish molecular cytogenetics


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HENNING, Frederico, Vladimir TRIFONOV, Lurdes Foresti de ALMEIDA-TOLEDO, 2008. Use of chromosome microdissection in fish molecular cytogenetics. In: Genetics and molecular biology. 31(1 suppl), pp. 279-283. Available under: doi: 10.1590/S1415-47572008000200022 

@article{Henning2008chrom-8007, title={Use of chromosome microdissection in fish molecular cytogenetics}, year={2008}, doi={10.1590/S1415-47572008000200022 }, number={1 suppl}, volume={31}, journal={Genetics and molecular biology}, pages={279--283}, author={Henning, Frederico and Trifonov, Vladimir and Almeida-Toledo, Lurdes Foresti de} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:contributor>Trifonov, Vladimir</dc:contributor> <dcterms:available rdf:datatype="">2011-03-24T17:39:14Z</dcterms:available> <dcterms:bibliographicCitation>First publ. in: Genetics and molecular biology 31 (2008), 1 suppl., pp. 279-283</dcterms:bibliographicCitation> <dc:contributor>Henning, Frederico</dc:contributor> <dc:creator>Almeida-Toledo, Lurdes Foresti de</dc:creator> <dc:creator>Henning, Frederico</dc:creator> <dcterms:rights rdf:resource=""/> <dc:date rdf:datatype="">2011-03-24T17:39:14Z</dc:date> <dcterms:title>Use of chromosome microdissection in fish molecular cytogenetics</dcterms:title> <dc:rights>terms-of-use</dc:rights> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dspace:hasBitstream rdf:resource=""/> <dc:format>application/pdf</dc:format> <dc:contributor>Almeida-Toledo, Lurdes Foresti de</dc:contributor> <dcterms:abstract xml:lang="eng">Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes.</dcterms:abstract> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> <dc:language>eng</dc:language> <dc:creator>Trifonov, Vladimir</dc:creator> <bibo:uri rdf:resource=""/> <dcterms:hasPart rdf:resource=""/> <dcterms:issued>2008</dcterms:issued> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> </rdf:Description> </rdf:RDF>

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