Mutants of Alcaligenes eutrophus Defective in Autotrophic Metabolism

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SCHINK, Bernhard, Hans G. SCHLEGEL, 1978. Mutants of Alcaligenes eutrophus Defective in Autotrophic Metabolism. In: Archives of Microbiology. 117(2), pp. 123-129. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00402299

@article{Schink1978Mutan-7974, title={Mutants of Alcaligenes eutrophus Defective in Autotrophic Metabolism}, year={1978}, doi={10.1007/BF00402299}, number={2}, volume={117}, issn={0302-8933}, journal={Archives of Microbiology}, pages={123--129}, author={Schink, Bernhard and Schlegel, Hans G.} }

terms-of-use eng Schink, Bernhard Schink, Bernhard 2011-03-24T17:38:58Z 2011-03-24T17:38:58Z Forty-four mutants of Alcaligenes eutrophus H 16 were isolated which grew poorly or not at all under autotrophic conditions. Four types were characterized with respect to their defects and their physiological properties. One mutant lacked both enzymes specific for autotrophic CO 2 fixation, another one lacked both hydrogenases, and two mutants lacked either the membrane-bound or the soluble hydrogenase.<br />Comparing the results of studies on these mutant types, the following conclusions were drawn: the lack of each hydrogenase enzyme could be partially compensated by the other one; the lack of membrane-bound hydrogenase did not affect autotrophic growth, whereas the lack of the soluble hydrogenase resulted in a decreased autotrophic growth rate. When pyruvate as well as hydrogen were supplied to the wild-type, the cell yield was higher than in the presence ofpyruvate alone.<br />Mutant experiments under these conditions indicated that either of both hydrogenases was able to add to the energy supply of the cell. Only the soluble hydrogenase was involved in the control of the rate of hydrogen oxidation by carbon dioxide; the mutant lacking this enzyme did not respond to the presence or absence of CO 2. The suppression of growth on fructose by hydrogen could be mediated by either of both hydrogenases alone. Schlegel, Hans G. First publ. in: Archives of Microbiology 117 (1978), pp. 123-129 1978 application/pdf Schlegel, Hans G. Mutants of Alcaligenes eutrophus Defective in Autotrophic Metabolism

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