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Calcium stores in differentiated Dictyostelium discoideum : prespore cells sequester calcium more efficiently than prestalk cells

Calcium stores in differentiated Dictyostelium discoideum : prespore cells sequester calcium more efficiently than prestalk cells

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SCHLATTERER, Christina, Paul WALTHER, M. MÜLLER, Kurt MENDGEN, Karl ZIEROLD, Gerd KNOLL, 2001. Calcium stores in differentiated Dictyostelium discoideum : prespore cells sequester calcium more efficiently than prestalk cells. In: Cell Calcium. 29(3), pp. 171-182. ISSN 0143-4160

@article{Schlatterer2001Calci-7951, title={Calcium stores in differentiated Dictyostelium discoideum : prespore cells sequester calcium more efficiently than prestalk cells}, year={2001}, doi={10.1054/ceca.2000.0181}, number={3}, volume={29}, issn={0143-4160}, journal={Cell Calcium}, pages={171--182}, author={Schlatterer, Christina and Walther, Paul and Müller, M. and Mendgen, Kurt and Zierold, Karl and Knoll, Gerd} }

Calcium stores in differentiated Dictyostelium discoideum : prespore cells sequester calcium more efficiently than prestalk cells 2011-03-24T17:38:47Z deposit-license Müller, M. Knoll, Gerd Schlatterer, Christina Mendgen, Kurt Walther, Paul eng application/pdf Müller, M. Mendgen, Kurt 2001 Dictyostelium discoideum pseudoplasmodia exhibit a gradient of the cytosolic free Ca2]-concentration ([Ca2]]i) along their anterior posterior axis involved in cell-type specific differentiation. [Ca2]]i is high in prestalk and low in prespore cells.We determined the content and localization of calcium and other elements in cryosectioned cells of pseudoplasmodia and fruiting bodies by X-ray microanalysis. Granular stores rich in Ca, Mg and P were identified. Average Ca was higher in prespore than prestalk granules (225 vs 111 mmol/kg dry weight). Total Ca stored in granules was also higher in prespore than prestalk cells. The amount of P and S in granules differed between the two cell types indicating different store composition. In spores mean granular Ca was 120 mmol/kg dry weight. Stalk cells had smaller granules with 360 mmol Ca/kg dry weight. Complementary to microanalysis, vesicular Ca2]-fluxes were studied in fractionated cell homogenates. The rate of Ca2]-uptake was higher in pellet fractions of prespore than prestalk amoebae (4.7 vs 3.4 nmol/minemg). Ca2]-release was greater in supernatant fractions from prestalk than prespore<br />cells (16.5 vs 7.7 nmol/108 cells). In summary, prestalk and prespore cells possess qualitatively different, high-capacity stores containing distinct amounts of Ca and probably being involved in regulation of the anterior posterior [Ca2]]i-gradient. Knoll, Gerd Zierold, Karl 2011-03-24T17:38:47Z Schlatterer, Christina Zierold, Karl Walther, Paul First publ. in: Cell Calcium 29 (2001), p. 171-182

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