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Identification and Properties of 8-Hydroxyflavin : Adenine Dinucleotide in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii

Identification and Properties of 8-Hydroxyflavin : Adenine Dinucleotide in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii

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GHISLA, Sandro, Stephen G. MAYHEW, 1976. Identification and Properties of 8-Hydroxyflavin : Adenine Dinucleotide in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii. In: European Journal of Biochemistry. 63(2), pp. 373-390. ISSN 0014-2956. eISSN 1432-1033

@article{Ghisla1976Ident-7903, title={Identification and Properties of 8-Hydroxyflavin : Adenine Dinucleotide in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii}, year={1976}, doi={10.1111/j.1432-1033.1976.tb10239.x}, number={2}, volume={63}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={373--390}, author={Ghisla, Sandro and Mayhew, Stephen G.} }

Ghisla, Sandro Mayhew, Stephen G. 2011-03-24T17:38:23Z 1. A new flavin prosthetic group has been isolated in pure form from the electron-transferring flavoprotein of Peptostreptococcus elsdenii. Its structure has been established as the FAD derivative of 7-methyl-8-hydroxyisoalloxazine.<br />Proof of this structure has been obtained by chemical synthesis of 7-methyl-8-hydroxyisoalloxazine models, and by stepwise degradation of the native compound to 7-methyl-8-hydroxyalloxazine. The orange chromophore is characterized by a strong absorption band with a maximum at 472 nm (ε = 41000 M-1 cm-1) and a pK at 4.8 due to the ionisation of the C(8)-OH group.<br /><br />2. The properties of a series of functionally substituted, derivatives of 8-hydroxy flavins and lumichromes have been investigated to provide a basis for interpreting the effects of pH on the spectroscopic properties of the 8-hydroxy derivatives of FAD and FMN.<br /><br />3. The 8-hydroxy derivative of FAD is bound by apo-d-amino acid oxidase; the complex shows no catalytic activity. The 8-hydroxy derivative of FMN is bound by apoflavodoxin to give a complex which has catalytic activity similar to that of native flavodoxin. The complex is reversibly reduced by dithionite, first to a relatively stable semiquinone and further to the dihydroflavin form. deposit-license eng application/pdf 1976 First publ. in: European Journal of Biochemistry 63 (1976), 2, pp. 373-390 Identification and Properties of 8-Hydroxyflavin : Adenine Dinucleotide in Electron-Transferring Flavoprotein from Peptostreptococcus elsdenii Ghisla, Sandro Mayhew, Stephen G. 2011-03-24T17:38:23Z

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