Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-39483 |
Author: | Krug, Michael; Lee, Sung-Jae; Diederichs, Kay; Boos, Winfried; Welte, Wolfram |
Year of publication: | 2006 |
Published in: | Journal of Biological Chemistry ; 281 (2006), 16. - pp. 10976-10982. - ISSN 0021-9258. - eISSN 1083-351X |
Pubmed ID: | 16473881 |
DOI (citable link): | https://dx.doi.org/10.1074/jbc.M512809200 |
Summary: |
TrmB is an alpha-glucoside-sensing transcriptional regulator controlling two operons encoding maltose/trehalose and maltodextrin ABC transporters of Pyrococcus furiosus. The crystal structure of an N-terminal truncated derivative of TrmB was solved at 1.5A resolution. This protein has lost its DNA binding domain but has retained its sugar recognition site. The structure represents a novel sugar-binding fold. TrmB bound maltose, glucose, sucrose, and maltotriose, exhibiting Kd values of 6.8, 25, 34, and 160 μM, respectively. TrmB behaved as a monomer in dilute buffer solution in contrast to the full-length protein, which is a dimer. Co-crystallization with bound maltose identified a binding site involving seven amino acid residues: Ser229, Asn305, Gly320, Met321, Val324, Ile325, and Glu326. Six of these residues interact with the nonreducing glucosyl residue of maltose. The nonreducing glucosyl residue is shared by all substrates bound to TrmB, suggesting it as a common recognition motif.
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Subject (DDC): | 570 Biosciences, Biology |
Link to License: | Attribution-NonCommercial-NoDerivs 2.0 Generic |
Bibliography of Konstanz: | Yes |
KRUG, Michael, Sung-Jae LEE, Kay DIEDERICHS, Winfried BOOS, Wolfram WELTE, 2006. Crystal Structure of the Sugar Binding Domain of the Archaeal Transcritional Regulator TrmB. In: Journal of Biological Chemistry. 281(16), pp. 10976-10982. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M512809200
@article{Krug2006Cryst-7898, title={Crystal Structure of the Sugar Binding Domain of the Archaeal Transcritional Regulator TrmB}, year={2006}, doi={10.1074/jbc.M512809200}, number={16}, volume={281}, issn={0021-9258}, journal={Journal of Biological Chemistry}, pages={10976--10982}, author={Krug, Michael and Lee, Sung-Jae and Diederichs, Kay and Boos, Winfried and Welte, Wolfram} }
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