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The oxygenase component of the2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1

The oxygenase component of the2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1

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MAMPEL, Jörg, Jürgen RUFF, Frank JUNKER, Alasdair M. COOK, 1999. The oxygenase component of the2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1. In: Microbiology. 145(11), pp. 3255-3264. ISSN 1350-0872. eISSN 1465-2080

@article{Mampel1999oxyge-7876, title={The oxygenase component of the2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1}, year={1999}, doi={10.1099/00221287-145-11-3255}, number={11}, volume={145}, issn={1350-0872}, journal={Microbiology}, pages={3255--3264}, author={Mampel, Jörg and Ruff, Jürgen and Junker, Frank and Cook, Alasdair M.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7876"> <dcterms:issued>1999</dcterms:issued> <dc:creator>Ruff, Jürgen</dc:creator> <dcterms:abstract xml:lang="eng">Growth of Alcaligenes sp. strain O-1 with 2-aminobenzenesulfonate (ABS; orthanilate) as sole source of carbon and energy requires expression of the soluble, multicomponent 2-aminobenzenesulfonate 2,3-dioxygenase system (deaminating) (ABSDOS) which is plasmid-encoded. ABSDOS was separated by anion-exchange chromatography to yield a flavin-dependent reductase component and an iron-dependent oxygenase component. The oxygenase component was purified to about 98% homogeneity and an {alpha}2ß2 subunit structure was deduced from the molecular masses of 134, 45 and 16 kDa for the native complex, and the {alpha} and ß subunits, respectively. Analysis of the amount of acid labile sulfur and total iron, and the UV spectrum of the purified oxygenase component indicated one [2Fe 2S] Rieske centre per {alpha} subunit. The inhibition of activity by the iron-specific chelator o-phenanthroline indicated the presence of an additional iron-binding site. Recovery of active protein required strictly anoxic conditions during all purification steps. The FAD-containing reductase could not be purified. ABSDOS oxygenated nine sulfonated compounds; no oxygen uptake was detected with carboxylated aromatic compounds or with aliphatic sulfonated compounds. Km values of 29, 18 and 108 µM and Vmax values of 140, 110 and 72 pkat for ABS, benzenesulfonate and 4-toluenesulfonate, respectively, were observed. The N-terminal amino acid sequences of the {alpha}- and ß-subunits of the oxygenase component allowed PCR primers to be deduced and the DNA sequence of the {alpha}-subunit was thereafter determined. Both redox centres were detected in the deduced amino acid sequence. Sequence data and biochemical properties of the enzyme system indicate a novel member of the class IB ring-hydroxylating dioxygenases.</dcterms:abstract> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:38:12Z</dcterms:available> <dc:rights>deposit-license</dc:rights> <dc:creator>Junker, Frank</dc:creator> <dcterms:bibliographicCitation>First publ. in: Microbiology 145 (1999), 11, pp. 3255-3264</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7876"/> <dc:contributor>Ruff, Jürgen</dc:contributor> <dc:contributor>Mampel, Jörg</dc:contributor> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dcterms:title>The oxygenase component of the2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1</dcterms:title> <dc:language>eng</dc:language> <dc:format>application/pdf</dc:format> <dc:contributor>Junker, Frank</dc:contributor> <dc:creator>Cook, Alasdair M.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:38:12Z</dc:date> <dc:creator>Mampel, Jörg</dc:creator> </rdf:Description> </rdf:RDF>

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