Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic

Domaszewicz, Wolfgang; Apell, Hans-Jürgen

Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic

Dateien

Binding_of_the_third.pdfGröße: 234.45 KBDownloads: 417

Datum

1999

Autor:innen

Domaszewicz, Wolfgang

Apell, Hans-Jürgen

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEBS Letters. 1999, 458(2), pp. 241-246. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(99)01162-X

Zusammenfassung

A new experimental setup was constructed to allow parallel measurements of total internal reflection fluorescence and of capacitance changes in Na,K-ATPase-containing membranes. Effects correlated with cytoplasmatic sodium binding to Na,K-ATPase were investigated. Ion binding-induced fluorescence changes of the electrochromic dye RH421 in membrane fragments adsorbed on a transparent capacitative electrode corresponded perfectly to capacitance increases detected by a lock-in technique. From these electric measurements it was possible to estimate a dielectric coefficient of about 0.25 for the electrogenetic binding of the third Na+ ion. Binding of K+ to cytoplasmic sites was electroneutral.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Sodium pump, Ion movement, Electrogenic binding, Fluorescent dye, Capacitative coupling

Zitieren

ISO 690

DOMASZEWICZ, Wolfgang, Hans-Jürgen APELL, 1999. Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic. In: FEBS Letters. 1999, 458(2), pp. 241-246. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(99)01162-X

BibTex

@article{Domaszewicz1999Bindi-7852,
  year={1999},
  doi={10.1016/S0014-5793(99)01162-X},
  title={Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic},
  number={2},
  volume={458},
  issn={0014-5793},
  journal={FEBS Letters},
  pages={241--246},
  author={Domaszewicz, Wolfgang and Apell, Hans-Jürgen}
}

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