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Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK

Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK

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VORDERWÜLBECKE, Sonja, Günter KRAMER, Frieder MERZ, Thorben A. KURZ, Thomas RAUCH, Beate ZACHMANN-BRAND, Bernd BUKAU, Elke DEUERLING, 2004. Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK. In: FEBS Letters. 559(1-3), pp. 181-187. ISSN 0014-5793

@article{Vorderwulbecke2004tempe-7841, title={Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK}, year={2004}, doi={10.1016/S0014-5793(04)00052-3}, number={1-3}, volume={559}, issn={0014-5793}, journal={FEBS Letters}, pages={181--187}, author={Vorderwülbecke, Sonja and Kramer, Günter and Merz, Frieder and Kurz, Thorben A. and Rauch, Thomas and Zachmann-Brand, Beate and Bukau, Bernd and Deuerling, Elke} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7841"> <dc:contributor>Deuerling, Elke</dc:contributor> <dc:contributor>Kurz, Thorben A.</dc:contributor> <dc:creator>Merz, Frieder</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:creator>Rauch, Thomas</dc:creator> <dc:creator>Vorderwülbecke, Sonja</dc:creator> <dc:contributor>Bukau, Bernd</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:format>application/pdf</dc:format> <dc:creator>Deuerling, Elke</dc:creator> <dcterms:title>Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK</dcterms:title> <dc:language>eng</dc:language> <dc:contributor>Zachmann-Brand, Beate</dc:contributor> <dc:contributor>Rauch, Thomas</dc:contributor> <dc:contributor>Vorderwülbecke, Sonja</dc:contributor> <dc:creator>Zachmann-Brand, Beate</dc:creator> <dc:creator>Kramer, Günter</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:56Z</dc:date> <dc:contributor>Merz, Frieder</dc:contributor> <dc:creator>Kurz, Thorben A.</dc:creator> <dc:contributor>Kramer, Günter</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7841"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:56Z</dcterms:available> <dcterms:issued>2004</dcterms:issued> <dcterms:abstract xml:lang="eng">Escherichia coli trigger factor (TF) and DnaK cooperate in the folding of newly synthesized proteins. The combined deletion of the TF-encoding tig gene and the dnaK gene causes protein aggregation and synthetic lethality at 30°C. Here we show that the synthetic lethality of ΔtigΔdnaK52 cells is abrogated either by growth below 30°C or by overproduction of GroEL/GroES. At 23°C ΔtigΔdnaK52 cells were viable and showed only minor protein aggregation. Overproduction of GroEL/GroES, but not of other chaperones, restored growth of ΔtigΔdnaK52 cells at 30°C and suppressed protein aggregation including proteins ≥60kDa, which normally require TF and DnaK for folding. GroEL/GroES thus influences the folding of proteins previously identified as DnaK/TF substrates.</dcterms:abstract> <dcterms:bibliographicCitation>First publ. in: FEBS Letters 559 (2004), pp. 181-187</dcterms:bibliographicCitation> <dc:creator>Bukau, Bernd</dc:creator> </rdf:Description> </rdf:RDF>

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