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No Nogo66- and NgR-Mediated Inhibition of Regenerating Axons in the Zebrafish Optic Nerve

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No Nogo66- and NgR-Mediated Inhibition of Regenerating Axons in the Zebrafish Optic Nerve

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ABDESSELEM, Houari, Aleksandra SHYPITSYNA, Gonzalo SOLIS PADILLA, Vsevolod BODRIKOV, Claudia STÜRMER, 2009. No Nogo66- and NgR-Mediated Inhibition of Regenerating Axons in the Zebrafish Optic Nerve. In: The Journal of Neuroscience. 29(49), pp. 15489-15498. ISSN 0270-6474. eISSN 1529-2401. Available under: doi: 10.1523/JNEUROSCI.3561-09.2009

@article{Abdesselem2009Nogo6-7835, title={No Nogo66- and NgR-Mediated Inhibition of Regenerating Axons in the Zebrafish Optic Nerve}, year={2009}, doi={10.1523/JNEUROSCI.3561-09.2009}, number={49}, volume={29}, issn={0270-6474}, journal={The Journal of Neuroscience}, pages={15489--15498}, author={Abdesselem, Houari and Shypitsyna, Aleksandra and Solis Padilla, Gonzalo and Bodrikov, Vsevolod and Stürmer, Claudia} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7835"> <dc:creator>Abdesselem, Houari</dc:creator> <dc:contributor>Solis Padilla, Gonzalo</dc:contributor> <dc:creator>Solis Padilla, Gonzalo</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7835/1/Abdesselem_No_nogo.pdf"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7835"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:title>No Nogo66- and NgR-Mediated Inhibition of Regenerating Axons in the Zebrafish Optic Nerve</dcterms:title> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Bodrikov, Vsevolod</dc:contributor> <dc:creator>Stürmer, Claudia</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:54Z</dcterms:available> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7835/1/Abdesselem_No_nogo.pdf"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:54Z</dc:date> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dcterms:bibliographicCitation>First publ. in: The Journal of Neuroscience 29 (2009), 49, pp. 15489-15498</dcterms:bibliographicCitation> <dc:format>application/pdf</dc:format> <dc:contributor>Abdesselem, Houari</dc:contributor> <dc:creator>Bodrikov, Vsevolod</dc:creator> <dc:contributor>Shypitsyna, Aleksandra</dc:contributor> <dc:creator>Shypitsyna, Aleksandra</dc:creator> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">In contrast to mammals, lesioned axons in the zebrafish (ZF) optic nerve regenerate and restore vision. This correlates with the absence of the NogoA-specific N-terminal domains from the ZF nogo/rtn-4 (reticulon-4) gene that inhibits regeneration in mammals. However, mammalian nogo/rtn-4 carries a second inhibitory C-terminal domain, Nogo-66, being 70% identical with ZF-Nogo66. The present study examines, (1) whether ZF-Nogo66 is inhibitory and effecting similar signaling pathways upon Nogo66-binding to the Nogo66 receptor NgR and its coreceptors, and (2) whether Rat-Nogo66 on fish, and ZF-Nogo66 on mouse neurons, cause inhibition via NgR. Our results from "outgrowth, collapse and contact assays" suggest, surprisingly, that ZF-Nogo66 is growth-permissive for ZF and mouse neurons, quite in contrast to its Rat-Nogo66 homolog which inhibits growth. The opposite effects of ZF- and Rat-Nogo66 are, in both fish and mouse, transmitted by GPI (glycosylphosphatidylinositol)-anchored receptors, including NgR. The high degree of sequence homology in the predicted binding site is consistent with the ability of ZF- and mammalian-Nogo66 to bind to NgRs of both species. Yet, Rat-Nogo66 elicits phosphorylation of the downstream effector cofilin whereas ZF-Nogo66 has no influence on cofilin phosphorylation probably because of significantly different Rat- versus ZF-Nogo66 sequences outside of the receptor-binding region effecting, by speculation, recruitment of a different set of coreceptors or microdomain association of NgR. Thus, not only was the NogoA-specific domain lost in fish, but Nogo66, the second inhibitory domain in mammals, and its signaling upon binding to NgR, was modified so that ZF-Nogo/RTN-4 does not impair axon regeneration.</dcterms:abstract> <dc:contributor>Stürmer, Claudia</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dcterms:issued>2009</dcterms:issued> </rdf:Description> </rdf:RDF>

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