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Photochemical behavior and Na+,K+-ATPase sensitivity of voltage-sensitive Styrylpyridinium fluorescent membrane probes

Photochemical behavior and Na+,K+-ATPase sensitivity of voltage-sensitive Styrylpyridinium fluorescent membrane probes

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AMOROSO, Steve, Vanessa V. AGON, Thomas STARKE-PETERKOVIC, Malcolm D. MCLEOD, Hans-Jürgen APELL, Pierre SEBBAN, Ronald J. CLARKE, 2006. Photochemical behavior and Na+,K+-ATPase sensitivity of voltage-sensitive Styrylpyridinium fluorescent membrane probes. In: Photochemistry and Photobiology. 82(2), pp. 495-502. ISSN 0031-8655

@article{Amoroso2006Photo-7798, title={Photochemical behavior and Na+,K+-ATPase sensitivity of voltage-sensitive Styrylpyridinium fluorescent membrane probes}, year={2006}, doi={10.1562/2005-06-08-RA-569}, number={2}, volume={82}, issn={0031-8655}, journal={Photochemistry and Photobiology}, pages={495--502}, author={Amoroso, Steve and Agon, Vanessa V. and Starke-Peterkovic, Thomas and McLeod, Malcolm D. and Apell, Hans-Jürgen and Sebban, Pierre and Clarke, Ronald J.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7798"> <dcterms:title>Photochemical behavior and Na+,K+-ATPase sensitivity of voltage-sensitive Styrylpyridinium fluorescent membrane probes</dcterms:title> <dc:rights>deposit-license</dc:rights> <dc:contributor>Sebban, Pierre</dc:contributor> <dc:creator>Amoroso, Steve</dc:creator> <dc:contributor>Clarke, Ronald J.</dc:contributor> <dcterms:abstract xml:lang="eng">RH421 is a widely used voltage-sensitive fluorescent membrane probe. Its exposure to continuous illumination with 577 nm light from an Hg lamp leads, however, to an increase in its steady-state fluorescence level when bound to lipid membranes. The increase occurs on the second time scale at typical light intensities and was found to be due to a single-photon excitedstate isomerization. Modifications to the dye structure are, therefore, necessary to increase photochemical stability and allow wider application of such dyes in kinetic studies of ion-transportingmembrane proteins. The related probe ANNINE 5, which has a rigid polycyclic struc ture, shows no observable photochemical reaction when bound to DMPC vesicles on irradiation with 436 nm light. The voltage sensitivity of ANNINE 5 was tested with the use of Na+,K+-ATPase membrane fragments. As long as ANNINE 5 is excited on the far red edge of its visible absorption band, it shows a similar sensitivity to RH421 in detecting charge-translocating reactions triggered by ATP phosphorylation. Unfortunately the wavelengths necessary for ANNINE 5 excitation are in a region where the Hg lamps routinely used in stopped-flow apparatus have no significant lines available for excitation.</dcterms:abstract> <dc:creator>Apell, Hans-Jürgen</dc:creator> <dc:format>application/pdf</dc:format> <dc:contributor>McLeod, Malcolm D.</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:issued>2006</dcterms:issued> <dc:creator>Starke-Peterkovic, Thomas</dc:creator> <dc:contributor>Agon, Vanessa V.</dc:contributor> <dc:creator>Clarke, Ronald J.</dc:creator> <dc:creator>McLeod, Malcolm D.</dc:creator> <dc:contributor>Apell, Hans-Jürgen</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Photochemistry and Photobiology 82 (2006), pp. 495-502</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:37Z</dcterms:available> <dc:contributor>Amoroso, Steve</dc:contributor> <dc:contributor>Starke-Peterkovic, Thomas</dc:contributor> <dc:creator>Agon, Vanessa V.</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7798"/> <dc:creator>Sebban, Pierre</dc:creator> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:37Z</dc:date> </rdf:Description> </rdf:RDF>

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