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Tributyltin-Induced Apoptosis Requires Glycolytic Adenosine Trisphosphate Production

Tributyltin-Induced Apoptosis Requires Glycolytic Adenosine Trisphosphate Production


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STRIDH, Hélène, Eugenio FAVA, Barbara SINGLE, Pierluigi NICOTERA, Sten ORRENIUS, Marcel LEIST, 1999. Tributyltin-Induced Apoptosis Requires Glycolytic Adenosine Trisphosphate Production. In: Chemical Research in Toxicology. 12(10), pp. 874-882. ISSN 0893-228X. eISSN 1520-5010

@article{Stridh1999Tribu-7792, title={Tributyltin-Induced Apoptosis Requires Glycolytic Adenosine Trisphosphate Production}, year={1999}, doi={10.1021/tx990041c}, number={10}, volume={12}, issn={0893-228X}, journal={Chemical Research in Toxicology}, pages={874--882}, author={Stridh, Hélène and Fava, Eugenio and Single, Barbara and Nicotera, Pierluigi and Orrenius, Sten and Leist, Marcel} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7792"> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7792"/> <dcterms:issued>1999</dcterms:issued> <dcterms:title>Tributyltin-Induced Apoptosis Requires Glycolytic Adenosine Trisphosphate Production</dcterms:title> <dcterms:bibliographicCitation>First publ. in: Chemical Research in Toxicology ; 12 (1999). - S. 874-882</dcterms:bibliographicCitation> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:34Z</dc:date> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">The toxicity of tributyltin chloride (TBT) involves Ca2+ overload, cytoskeletal damage, and mitochondrial failure leading to cell death by apoptosis or necrosis. Here, we examined whether the intracellular ATP level modulates the mode of cell death after exposure to TBT. When Jurkat cells were energized by the mitochondrial substrate, pyruvate, low concentrations of TBT (1-2 íM) triggered an immediate depletion of intracellular ATP followed by necrotic death. When ATP levels were maintained by the addition of glucose, the mode of cell death was typically apoptotic. Glycolytic ATP production was required for apoptosis at two distinct steps. First, maintenance of adequate ATP levels accelerated the decrease of mitochondrial membrane potential, and the release of the intermembrane proteins adenylate kinase and cytochrome c from mitochondria. A possible role of the adenine nucleotide exchanger in this first ATPdependent step is suggested by experiments performed with the specific inhibitor, bongkrekic acid. This substance delayed cytochrome c release in a manner similar to that caused by ATP depletion. Second, caspase activation following cytochrome c release was only observed in ATPcontaining cells. Bcl-2 had only a minor effect on TBT-triggered caspase activation or cell death. We conclude that intracellular ATP concentrations control the mode of cell death in TBTtreated Jurkat cells at both the mitochondrial and caspase activation levels.</dcterms:abstract> <dc:creator>Single, Barbara</dc:creator> <dc:creator>Leist, Marcel</dc:creator> <dc:contributor>Single, Barbara</dc:contributor> <dc:contributor>Leist, Marcel</dc:contributor> <dc:contributor>Fava, Eugenio</dc:contributor> <dc:creator>Nicotera, Pierluigi</dc:creator> <dc:contributor>Nicotera, Pierluigi</dc:contributor> <dc:creator>Stridh, Hélène</dc:creator> <dc:creator>Fava, Eugenio</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:contributor>Orrenius, Sten</dc:contributor> <dc:contributor>Stridh, Hélène</dc:contributor> <dc:creator>Orrenius, Sten</dc:creator> <dc:format>application/pdf</dc:format> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> </rdf:Description> </rdf:RDF>

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