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14C02 exchange with acetoacetate catalyzed by dialyzed cell-free extracts of the bacterial strain BunN grown with acetone and nitrate

14C02 exchange with acetoacetate catalyzed by dialyzed cell-free extracts of the bacterial strain BunN grown with acetone and nitrate

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JANSSEN, Peter H., Bernhard SCHINK, 1995. 14C02 exchange with acetoacetate catalyzed by dialyzed cell-free extracts of the bacterial strain BunN grown with acetone and nitrate. In: European Journal of Biochemistry. 228(3), pp. 677-682. ISSN 0014-2956. eISSN 1432-1033

@article{Janssen199514C02-7748, title={14C02 exchange with acetoacetate catalyzed by dialyzed cell-free extracts of the bacterial strain BunN grown with acetone and nitrate}, year={1995}, doi={10.1111/j.1432-1033.1995.0677m.x}, number={3}, volume={228}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={677--682}, author={Janssen, Peter H. and Schink, Bernhard} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7748"> <dc:contributor>Schink, Bernhard</dc:contributor> <dcterms:abstract xml:lang="eng">The nitrate-reducing bacterial strain BunN is able to grow with acetone and nitrate under anoxic conditions. Dialyzed crude cell-free extracts of acetone-plus-nitrate-grown cells of strain BunN catalyzed the exchange of 14C0, into acetoacetate in an ADP-dependent reaction. The rates of exchange catalyzed by extracts of acetate-grown or 3-hydroxybutyrate-grown cells were only 13% of that catalyzed by extracts of acetone-grown cells. The activity was enzymic since it was destroyed by boiling and was proportional to the amount of added extract. The optimal acetoacetate concentration was 100 mM and the apparent K,,, was 11.1 mM. The pH optimum was 6.5, the exchange was not dependent on the addition of biotin, and the activity was not inhibited by avidin. The exchange activity was not stimulated (less than two fold) by a variety of metal ions or by a range of possible cofactors. Under optimal conditions (100 mM acetoacetate, 5 mM ADP, 10mh4 NaHC03, pH 6.5, under N,), the exchange activity was 2.7 nmol min-' . mg protein-'; 2% of the in vivo carboxylation activity of acetone-plus-nitrate-grown cultures. It is suggested that the exchange reaction is a partial reaction catalyzed by the enzyme (or enzyme complex) that carboxylates acetone, and that the methods developed in this study provide a means with which to investigate this reaction further.</dcterms:abstract> <dcterms:title>14C02 exchange with acetoacetate catalyzed by dialyzed cell-free extracts of the bacterial strain BunN grown with acetone and nitrate</dcterms:title> <dc:format>application/pdf</dc:format> <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 228 (1995), 3, pp. 677-682</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:rights>deposit-license</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:14Z</dcterms:available> <dc:contributor>Janssen, Peter H.</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7748"/> <dc:creator>Schink, Bernhard</dc:creator> <dc:creator>Janssen, Peter H.</dc:creator> <dc:language>eng</dc:language> <dcterms:issued>1995</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:14Z</dc:date> </rdf:Description> </rdf:RDF>

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