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Biosynthesis of tetrahydrobiopterin : purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver

Biosynthesis of tetrahydrobiopterin : purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver

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TAKIKAWA, Shin-Ichiro, Hans-Christoph CURTIUS, Udo REDWEIK, Walter LEIMBACHER, Sandro GHISLA, 1986. Biosynthesis of tetrahydrobiopterin : purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver. In: European Journal of Biochemistry. 161(2), pp. 295-302. ISSN 0014-2956. eISSN 1432-1033

@article{Takikawa1986Biosy-7740, title={Biosynthesis of tetrahydrobiopterin : purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver}, year={1986}, doi={10.1111/j.1432-1033.1986.tb10446.x}, number={2}, volume={161}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={295--302}, author={Takikawa, Shin-Ichiro and Curtius, Hans-Christoph and Redweik, Udo and Leimbacher, Walter and Ghisla, Sandro} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7740"> <dc:creator>Leimbacher, Walter</dc:creator> <dc:contributor>Takikawa, Shin-Ichiro</dc:contributor> <dc:creator>Redweik, Udo</dc:creator> <dc:format>application/pdf</dc:format> <dc:contributor>Leimbacher, Walter</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:11Z</dcterms:available> <dcterms:title>Biosynthesis of tetrahydrobiopterin : purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver</dcterms:title> <dc:creator>Takikawa, Shin-Ichiro</dc:creator> <dc:contributor>Redweik, Udo</dc:contributor> <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 161 (1986), 2, pp. 295-302</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7740"/> <dc:language>eng</dc:language> <dc:creator>Ghisla, Sandro</dc:creator> <dc:contributor>Curtius, Hans-Christoph</dc:contributor> <dcterms:abstract xml:lang="eng">6-Pyruvoyl-tetrahydropterin synthase, which catalyzes the first step in the conversion of 7,8-dihydroneopterin triphosphate to tetrahydrobiopterin, was purified approximately 140,000-fold to apparent homogeneity from human liver. The molecular mass of the enzyme is estimated to be 83 kDa. 7,8-Dihydroneopterin triphosphate was a substrate of the enzyme in the presence of Mg2+, and the pH optimum of the reaction was 7.5 in Tris HCl buffer. The Km value for 7,8-dihydroneopterin triphosphate was 10 μM. The product of this enzymatic reaction was the presumed intermediate 6-pyruvoyl-tetrahydropterin. This latter compound was converted to tetrahydrobiopterin in the presence of NADPH and partially purified sepiapterin reductase from human liver. The conditions and the effect of N-acetyserotonin on this reaction, and on the formation of the intermediates 6-(1'-hydroxy-2'-oxopropyl)-tetrahydropterin and 6-(1'oxo-2'-hydroxypropyl)-tetrahydropterin have been studied.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:11Z</dc:date> <dc:rights>deposit-license</dc:rights> <dc:creator>Curtius, Hans-Christoph</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:issued>1986</dcterms:issued> <dc:contributor>Ghisla, Sandro</dc:contributor> </rdf:Description> </rdf:RDF>

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