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Physikalische Kartierung und Genexpressionsanalysen im Genom von Neurospora crassa

Physikalische Kartierung und Genexpressionsanalysen im Genom von Neurospora crassa

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Prüfsumme: MD5:cebf7f9f43d87c899de6f8a889bc6456

AIGN, Verena, 2001. Physikalische Kartierung und Genexpressionsanalysen im Genom von Neurospora crassa [Dissertation]. Konstanz: University of Konstanz

@phdthesis{Aign2001Physi-7719, title={Physikalische Kartierung und Genexpressionsanalysen im Genom von Neurospora crassa}, year={2001}, author={Aign, Verena}, address={Konstanz}, school={Universität Konstanz} }

Physikalische Kartierung und Genexpressionsanalysen im Genom von Neurospora crassa Physical mapping and gene expression analysis in the genome of Neurospora crassa deu Aign, Verena application/pdf The filamentous fungus Neurospora crassa has been used as a model organism in basic science for more than 50 years. In germany, a neurospora genome project started in 1998 that aims at the sequencing of the chromosomes II and V.<br />The physical maps described in the first part of this work formed the basis of the sequencing project. For mapping, a cosmid library with a 17-fold genomic coverage and a BAC library with a 15-fold coverage were used, from which chromosome specific sublibraries were selected. Physical mapping was done by hybridising labelled DNA of single clones to the libraries. Ordering of the clones according to their hybridisation pattern was performed with an established software package. The resulting map of chromosome II contains 13 regions, the map of chromosome V 21 regions of overlapping clones (contigs).<br />In the second part of the work some gene expression profiling experiments were performed using selfmade cDNA microarrays of N. crassa. By hybridising complex RNA probes transcriptional profiles were generated for three different growth conditions of mycelium of N. crassa. In each experiment, two mRNA populations were labelled by reverse transkription with two different fluorescent dyes and cohybridised to a single chip. After detection and quantification of the signal intensities, factors for induction or repression of the genes were calculated and visualised in a correspondence analysis. 2011-03-24T17:36:38Z deposit-license 2001 2011-03-24T17:36:38Z Aign, Verena

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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