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Cysteine-mediated electron transfer in syntrophic acetate oxidation by cocultures of Geobacter sulfurreducens and Wolinella succinogenes

Cysteine-mediated electron transfer in syntrophic acetate oxidation by cocultures of Geobacter sulfurreducens and Wolinella succinogenes

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KADEN, Jan, Alexander S. GALUSHKO, Bernhard SCHINK, 2002. Cysteine-mediated electron transfer in syntrophic acetate oxidation by cocultures of Geobacter sulfurreducens and Wolinella succinogenes. In: Archives of Microbiology. 178(1), pp. 53-58. ISSN 0302-8933. eISSN 1432-072X

@article{Kaden2002Cyste-7688, title={Cysteine-mediated electron transfer in syntrophic acetate oxidation by cocultures of Geobacter sulfurreducens and Wolinella succinogenes}, year={2002}, number={1}, volume={178}, issn={0302-8933}, journal={Archives of Microbiology}, pages={53--58}, author={Kaden, Jan and Galushko, Alexander S. and Schink, Bernhard} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7688"> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:rights>deposit-license</dc:rights> <dc:language>eng</dc:language> <dc:creator>Schink, Bernhard</dc:creator> <dcterms:issued>2002</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:24Z</dc:date> <dc:contributor>Kaden, Jan</dc:contributor> <dcterms:title>Cysteine-mediated electron transfer in syntrophic acetate oxidation by cocultures of Geobacter sulfurreducens and Wolinella succinogenes</dcterms:title> <dc:format>application/pdf</dc:format> <dcterms:abstract xml:lang="eng">Syntrophic cocultures of Geobacter sulfurreducens and Wolinella succinogenes oxidize acetate with nitrate as terminal electron acceptor. It has been postulated earlier that electrons are transferred in these cocultures not via hydrogen, but via a different carrier, e.g., a small c-type cytochrome that is detected in the supernatant of growing cultures. In the present study, L-cysteine, which was provided as a reducing agent, was found to mediate the electron transfer between the two partners. Low concentrations of L-cysteine or L-cystine (10 100 μM) supported syntrophic growth, and no acetate oxidation was observed in the absence of cysteine or cystine. Cell suspensions of G. sulfurreducens or coculture cell suspensions reduced cystine to cysteine, and suspensions of W. succinogenes or coculture suspensions oxidized cysteine with nitrate, as measured by the formation or depletion of free thiol groups. Added cysteine was rapidly oxidized by the coculture during growth, but the formed cystine was not entirely rereduced even under acceptor-limited conditions. The redox potential prevailing in acetateoxidizing cocultures was 160 to 230 mV. Sulfide at low concentrations supported syntrophic growth as well and could replace cysteine. Neither growth nor acetate degradation was found with D-cysteine, homocysteine, cysteamine, 3-mercaptopropionate, dithiothreithol, thioglycolate, glutathione, coenzyme M, dimethylsulfoxide, trimethylamine-N-oxide, anthraquinone-2,6-disulfonate, or ascorbate.</dcterms:abstract> <dc:creator>Kaden, Jan</dc:creator> <dc:contributor>Schink, Bernhard</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:24Z</dcterms:available> <dc:creator>Galushko, Alexander S.</dc:creator> <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 178 (2000), 1, pp. 53-58</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7688"/> <dc:contributor>Galushko, Alexander S.</dc:contributor> </rdf:Description> </rdf:RDF>

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