A semi-in-vivo preparation for optical recording of the insect brain


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GALIZIA, Cosmas Giovanni, Jasdan JOERGES, Armin KÜTTNER, Till FABER, Randolf MENZEL, 1997. A semi-in-vivo preparation for optical recording of the insect brain. In: Journal of Neuroscience Methods. 76(1), pp. 61-69. ISSN 0165-0270

@article{Galizia1997semi--7654, title={A semi-in-vivo preparation for optical recording of the insect brain}, year={1997}, doi={10.1016/S0165-0270(97)00080-0}, number={1}, volume={76}, issn={0165-0270}, journal={Journal of Neuroscience Methods}, pages={61--69}, author={Galizia, Cosmas Giovanni and Joerges, Jasdan and Küttner, Armin and Faber, Till and Menzel, Randolf} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7654"> <dc:contributor>Küttner, Armin</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7654"/> <dc:creator>Menzel, Randolf</dc:creator> <dcterms:title>A semi-in-vivo preparation for optical recording of the insect brain</dcterms:title> <dc:creator>Faber, Till</dc:creator> <dc:creator>Küttner, Armin</dc:creator> <dcterms:bibliographicCitation>First publ. in: Journal of Neuroscience Methods 76 (1997), pp.61-69</dcterms:bibliographicCitation> <dc:creator>Joerges, Jasdan</dc:creator> <dcterms:abstract xml:lang="eng">We describe a method for optically recording neuronal activity from an intact insect brain, upon natural sensory stimulation. In this preparation, the head capsule of the honeybee, Apis mellifera, is isolated from the body while leaving the entire brain undamaged. In short, a hole is first cut into the cuticule to allow optical access to the brain and to allow the removal of tracheae and glands. Then the head is cut free and placed into a dye-loaded and cooled ringer solution in a staining chamber for 1 h. Subsequently, the head is fixed in a recording chamber, covered with a cover-slip, and imaged under the microscope with a cooled CCD camera. The whole preparation leaves the antennae dry, free to move, and functional throughout the experiment, allowing for natural odour stimulation of the olfactory system. Using calcium sensitive or potential sensitive dyes (calcium-green or RH795), we could record the processing of olfactory information at the glomerular level in the antennal lobe of the bee.</dcterms:abstract> <dc:creator>Galizia, Cosmas Giovanni</dc:creator> <dc:language>deu</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:06Z</dc:date> <dc:contributor>Faber, Till</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:06Z</dcterms:available> <dc:contributor>Menzel, Randolf</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:rights>deposit-license</dc:rights> <dc:contributor>Galizia, Cosmas Giovanni</dc:contributor> <dcterms:issued>1997</dcterms:issued> <dc:format>application/pdf</dc:format> <dc:contributor>Joerges, Jasdan</dc:contributor> </rdf:Description> </rdf:RDF>

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