Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains

Homann, Thomas; Tag, Carmen; Biebl, Hanno; Deckwer, Wolf-Dieter; Schink, Bernhard

Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains

Dateien

Fermentation_of_glycerol.pdfGröße: 826.95 KBDownloads: 1040

Datum

1990

Autor:innen

Homann, Thomas

Tag, Carmen

Biebl, Hanno

Deckwer, Wolf-Dieter

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Applied and microbiology biotechnology. 1990, 33(2), pp. 121-126. ISSN 0175-7598. eISSN 1432-0614. Available under: doi: 10.1007/BF00176511

Zusammenfassung

Glycerol-fermenting anaerobes were enriched with glycerol at low and high concentrations in order to obtain strains that produce 1,3-propanediol. Six isolates were selected for more detailed characterization; four of them were identified as Citrobacter freundii, one as Klebsiella oxytoca and one as K. pneumoniae. The Citrobacter strains formed 1,3-propanediol and acetate and almost no by-products, while the Klebsiella strains produced varying amounts of ethanol in addition and accordingly less 1,3-propanediol. Enterobacterial strains of the genera Enterobacter, Klebsiella, and Citrobacter from culture collections showed similar product patterns except for one group which formed limited amounts of ethanol, but no propanediol. Seven strains were grown in pH-controlled batch cultures to determine the parameters necessary to evaluate their capacity for 1,3-propanediol production. K. pneumoniae DSM 2026 exhibited the highest final concentration (61 g/l) and the best productivity (1.7 g/1 h) whereas C. freundii Zu and K2 achieved only 35 g/1 and 1.4 g/1 h, respectively. The Citrobacter strains on the other hand gave somewhat better yields which were very close to the theoretical optimum of 65 mol %.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

HOMANN, Thomas, Carmen TAG, Hanno BIEBL, Wolf-Dieter DECKWER, Bernhard SCHINK, 1990. Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains. In: Applied and microbiology biotechnology. 1990, 33(2), pp. 121-126. ISSN 0175-7598. eISSN 1432-0614. Available under: doi: 10.1007/BF00176511

BibTex

@article{Homann1990Ferme-7599,
  year={1990},
  doi={10.1007/BF00176511},
  title={Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains},
  number={2},
  volume={33},
  issn={0175-7598},
  journal={Applied and microbiology biotechnology},
  pages={121--126},
  author={Homann, Thomas and Tag, Carmen and Biebl, Hanno and Deckwer, Wolf-Dieter and Schink, Bernhard}
}

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