Simultaneous release of adenylate kinase and cytochrome c in cell death


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SINGLE, Barbara, Marcel LEIST, Pierluigi NICOTERA, 1998. Simultaneous release of adenylate kinase and cytochrome c in cell death. In: Cell Death and Differentiation. 12(5), pp. 1001-1003. ISSN 1350-9047. eISSN 1350-9047

@article{Single1998Simul-7598, title={Simultaneous release of adenylate kinase and cytochrome c in cell death}, year={1998}, doi={10.1038/sj.cdd.4400462}, number={5}, volume={12}, issn={1350-9047}, journal={Cell Death and Differentiation}, pages={1001--1003}, author={Single, Barbara and Leist, Marcel and Nicotera, Pierluigi} }

Cytochrome c (Cyt c) is located within the mitochondrial intermembrane space, and it is an essential constituent of the respiratory chain. The translocation of Cyt c from mitochondria to the cytosol is an important step in the apoptotic signaling pathway, linking mitochondrial changes to the activation of execution caspases (Liu et al, 1996). Once translocated into the cytosol, Cyt c together with Apaf-1 and procaspase-9 form a multiprotein complex, the apoptosome, which initiates the activation of caspase-3 (Li P et al, 1997). Studies in cell-free sytems have shown that addition of Cyt c to cytosols directly activates caspase-3, whereas immunodepletion of Cyt c from cell homogenates prevents caspase-3 activation. Furthermore, microinjection of Cyt c into intact cells has been shown to induce apoptosis (Li F et al, 1997). Although Cyt c release has been observed in many experimental models of apoptosis, the mechanism of translocation across the outer mitochondrial membrane is unclear. One question is whether Cyt c release occurs by a selective transport mechanism into the cytosol, or by a nonselective change of permeability in the outer mitochondrial<br />membrane. To distinguish between these two possibilities, we monitored the translocation of Cyt c and of the mitochondrial intermembrane space marker adenylate kinase (ADK). Nicotera, Pierluigi Leist, Marcel Leist, Marcel 2011-03-24T17:35:41Z deposit-license First publ. in: Cell Death and Differentiation 12 (1998), 5, pp. 1001-1003 eng Simultaneous release of adenylate kinase and cytochrome c in cell death Single, Barbara Nicotera, Pierluigi application/pdf 2011-03-24T17:35:41Z Single, Barbara 1998

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