KOPS - Das Institutionelle Repositorium der Universität Konstanz

Guanylyl cyclases with the topology of mammalian adenylyl cyclases and an N-terminal P-type ATPase-like domain in Paramecium, Tetrahymena and Plasmodium

Guanylyl cyclases with the topology of mammalian adenylyl cyclases and an N-terminal P-type ATPase-like domain in Paramecium, Tetrahymena and Plasmodium

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:502949f26caed512416c8cbb8428f846

LINDER, Jürgen, Peter ENGEL, Andreas REIMER, Thomas KRÜGER, Helmut PLATTNER, Anita SCHULTZ, Joachim E. SCHULTZ, 1999. Guanylyl cyclases with the topology of mammalian adenylyl cyclases and an N-terminal P-type ATPase-like domain in Paramecium, Tetrahymena and Plasmodium. In: The EMBO Journal. 18(15), pp. 4222-4232. ISSN 0261-4189. eISSN 1460-2075. Available under: doi: 10.1093/emboj/18.15.4222

@article{Linder1999Guany-7591, title={Guanylyl cyclases with the topology of mammalian adenylyl cyclases and an N-terminal P-type ATPase-like domain in Paramecium, Tetrahymena and Plasmodium}, year={1999}, doi={10.1093/emboj/18.15.4222}, number={15}, volume={18}, issn={0261-4189}, journal={The EMBO Journal}, pages={4222--4232}, author={Linder, Jürgen and Engel, Peter and Reimer, Andreas and Krüger, Thomas and Plattner, Helmut and Schultz, Anita and Schultz, Joachim E.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7591"> <dc:rights>deposit-license</dc:rights> <dc:creator>Schultz, Joachim E.</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7591/1/Guanylyl_cyclases_with_the_topology_of_mammalian_adenylyl_.pdf"/> <dc:language>eng</dc:language> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Schultz, Joachim E.</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:format>application/pdf</dc:format> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:abstract xml:lang="deu">We cloned a guanylyl cyclase of 280 kDa from the ciliate Paramecium which has an N-terminus similar to that of a P-type ATPase and a C-terminus with a topology identical to mammalian adenylyl cyclases. Respective signature sequence motifs are conserved in both domains. The cytosolic catalytic C1a and C2a segments of the cyclase are inverted. Genes coding for topologically identical proteins with substantial sequence similarities have been cloned from Tetrahymena and were detected in sequences from Plasmodium deposited by the Malaria Genome Project. After 99 point mutations to convert the Paramecium TAA/TAG-Gln triplets to CAA/CAG, together with partial gene synthesis, the gene from Paramecium was heterologously expressed. In Sf9 cells, the holoenzyme is proteolytically processed into the two domains. Immunocytochemistry demonstrates expression of the protein in Paramecium and localizes it to cell surface membranes. The data provide a novel structural link between class III adenylyl and guanylyl cyclases and imply that the protozoan guanylyl cyclases evolved from an ancestral adenylyl cyclase independently of the mammalian guanylyl cyclase isoforms. Further, signal transmission in Ciliophora (Paramecium, Tetrahymena) and in the most important endoparasitic phylum Apicomplexa (Plasmodium) is, quite unexpectedly, closely related.</dcterms:abstract> <dcterms:title>Guanylyl cyclases with the topology of mammalian adenylyl cyclases and an N-terminal P-type ATPase-like domain in Paramecium, Tetrahymena and Plasmodium</dcterms:title> <dc:creator>Schultz, Anita</dc:creator> <dc:creator>Linder, Jürgen</dc:creator> <dc:creator>Krüger, Thomas</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7591"/> <dcterms:issued>1999</dcterms:issued> <dc:contributor>Schultz, Anita</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:37Z</dc:date> <dc:creator>Reimer, Andreas</dc:creator> <dc:contributor>Krüger, Thomas</dc:contributor> <dc:creator>Plattner, Helmut</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Engel, Peter</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Linder, Jürgen</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7591/1/Guanylyl_cyclases_with_the_topology_of_mammalian_adenylyl_.pdf"/> <dc:contributor>Engel, Peter</dc:contributor> <dc:contributor>Plattner, Helmut</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:37Z</dcterms:available> <dcterms:bibliographicCitation>First publ. in: The EMBO Journal 18 (1999), 15, pp. 4222-4232</dcterms:bibliographicCitation> <dc:contributor>Reimer, Andreas</dc:contributor> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Guanylyl_cyclases_with_the_topology_of_mammalian_adenylyl_.pdf 172

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto