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Characterization of a novel NADP+-dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae

Characterization of a novel NADP+-dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae

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LINK, Tobias, Gertrud LOHAUS, Ingrid HEISER, Kurt MENDGEN, Matthias HAHN, Ralf T. VOEGELE, 2005. Characterization of a novel NADP+-dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae. In: Biochem. J. 389(2), pp. 289-295. ISSN 0264-6021. eISSN 1470-8728. Available under: doi: 10.1042/BJ20050301

@article{Link2005Chara-7590, title={Characterization of a novel NADP+-dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae}, year={2005}, doi={10.1042/BJ20050301}, number={2}, volume={389}, issn={0264-6021}, journal={Biochem. J}, pages={289--295}, author={Link, Tobias and Lohaus, Gertrud and Heiser, Ingrid and Mendgen, Kurt and Hahn, Matthias and Voegele, Ralf T.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7590"> <dc:creator>Voegele, Ralf T.</dc:creator> <dc:creator>Lohaus, Gertrud</dc:creator> <dc:contributor>Voegele, Ralf T.</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Biochem. J. 389 (2005), 289-295</dcterms:bibliographicCitation> <dc:format>application/pdf</dc:format> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:36Z</dcterms:available> <dc:language>eng</dc:language> <dc:contributor>Link, Tobias</dc:contributor> <dc:contributor>Heiser, Ingrid</dc:contributor> <dc:creator>Heiser, Ingrid</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Mendgen, Kurt</dc:contributor> <dc:creator>Hahn, Matthias</dc:creator> <dcterms:abstract xml:lang="eng">We have identified and characterized a novel NADP+-dependent D-arabitol dehydrogenase and the corresponding gene from the rust fungus Uromyces fabae, a biotrophic plant pathogen on broad bean (Vicia faba). The new enzyme was termed ARD1p (Darabitol dehydrogenase 1). It recognizes D-arabitol and mannitol as substrates in the forward reaction, and D-xylulose, D-ribulose and D-fructose as substrates in the reverse reaction. Co-factor specificity was restricted to NADP(H). Kinetic data for the major substrates and co-factors are presented. A detailed analysis of the organization and expression pattern of the ARD1 gene are also given. Immunocytological data indicate a localization of the gene product predominantly in haustoria, the feeding structures of these fungi. Analyses of metabolite levels during pathogenesis indicate that the D-arabitol concentration rises dramatically as infection progresses, and D-arabitol was shown in an in vitro system to be capable of quenching reactive oxygen species involved in host plant defence reactions. ARD1p may therefore play an important role in carbohydrate metabolism and in establishing and/or maintaining the biotrophic interaction in U. fabae.</dcterms:abstract> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7590/1/200_2005_Link.pdf"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:36Z</dc:date> <dc:creator>Mendgen, Kurt</dc:creator> <dcterms:issued>2005</dcterms:issued> <dc:creator>Link, Tobias</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7590"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7590/1/200_2005_Link.pdf"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Lohaus, Gertrud</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:contributor>Hahn, Matthias</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:title>Characterization of a novel NADP+-dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae</dcterms:title> </rdf:Description> </rdf:RDF>

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