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Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements

Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements

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SOKOLOV, Valerij S., Artem G. AYUYAN, Hans-Jürgen APELL, 2001. Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements. In: European Biophysics Journal. 30(7), pp. 515-527. ISSN 0175-7571. eISSN 1432-1017. Available under: doi: 10.1007/s002490100179

@article{Sokolov2001Assig-7585, title={Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements}, year={2001}, doi={10.1007/s002490100179}, number={7}, volume={30}, issn={0175-7571}, journal={European Biophysics Journal}, pages={515--527}, author={Sokolov, Valerij S. and Ayuyan, Artem G. and Apell, Hans-Jürgen} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7585"> <dcterms:issued>2001</dcterms:issued> <dcterms:bibliographicCitation>First publ. in: European Biophysics Journal 30 (2001), pp. 515-527</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:34Z</dcterms:available> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Ayuyan, Artem G.</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:language>eng</dc:language> <dc:creator>Apell, Hans-Jürgen</dc:creator> <dc:creator>Sokolov, Valerij S.</dc:creator> <dc:contributor>Sokolov, Valerij S.</dc:contributor> <dc:contributor>Apell, Hans-Jürgen</dc:contributor> <dc:creator>Ayuyan, Artem G.</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7585/1/Assignment_of_charge_movements_to_electrogenic_reaction_steps.pdf"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7585/1/Assignment_of_charge_movements_to_electrogenic_reaction_steps.pdf"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:34Z</dc:date> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7585"/> <dc:rights>terms-of-use</dc:rights> <dc:format>application/pdf</dc:format> <dcterms:abstract xml:lang="eng">Na,K-ATPase-enriched membrane fragments adsorbed to lipid bilayers were used to study electrogenic Na+ movements induced by anzyme phosphorylation when ATP was photo-relased from inactive caged ATP, and simultaneously by externally applied alterating voltages which allowed the measurement of small ATP-induced membrane admittance changes. A detailed analysis of frequency dependence of the capacitance and conductance increments showed that the observed peocess consists of more than one electrogenic step. The frequency dependence could be described by the sum of two Lorentzian functions and y constant term. Preferentially, the slower process showed a dependence on the ion concentration of choline salts with different anions. This observation matches their effect on the partition between two phosphoenzyme states of the Na,K-ATPase, as established previously.</dcterms:abstract> <dcterms:title>Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements</dcterms:title> </rdf:Description> </rdf:RDF>

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