KOPS - Das Institutionelle Repositorium der Universität Konstanz

Suicide inactivation of the flavoenzyme D-lactate dehydrogenase by alpha-hydroxybutynoate

Suicide inactivation of the flavoenzyme D-lactate dehydrogenase by alpha-hydroxybutynoate

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:cf94178b9a27fc4d401d6163641a4c99

OLSON, Steven T., Vincent MASSEY, Sandro GHISLA, Carolyn D. WHITFIELD, 1979. Suicide inactivation of the flavoenzyme D-lactate dehydrogenase by alpha-hydroxybutynoate. In: Biochemistry. 18(21), pp. 4724-4732. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi00588a037

@article{Olson1979Suici-7545, title={Suicide inactivation of the flavoenzyme D-lactate dehydrogenase by alpha-hydroxybutynoate}, year={1979}, doi={10.1021/bi00588a037}, number={21}, volume={18}, issn={0006-2960}, journal={Biochemistry}, pages={4724--4732}, author={Olson, Steven T. and Massey, Vincent and Ghisla, Sandro and Whitfield, Carolyn D.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7545"> <dc:creator>Whitfield, Carolyn D.</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7545/1/Suicide_inactivation_of_the_flavoenzyme_D_lactate_dehydrogenase_by_alpha_hydroxybutynoate.pdf"/> <dc:contributor>Ghisla, Sandro</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:18Z</dcterms:available> <dcterms:abstract xml:lang="eng">The acetylenic α-hydroxy acid 2-hydroxy-3- butynoate (αHB) is a substrate and an irreversible inactivator of the FAD-containing flavoenzyme D-lactate dehydrogenase from Megasphaera elsdenii. On the average, the enzyme undergoes five catalytic turnovers with αHB in air at pH 7.0 before being inactivated. Irreversible inactivation is due to the conversion of the flavin to a pink adduct with visible absorption peaks at 522, 382, and 330 nm and weak fluorescence with an emission maximum at 635 nm. The adduct is stable and can be released from the enzyme and purified. It retains a structure analogous to FAD since it binds to the FAD-specific apo-D-amino acid oxidase. It can be further converted to an FMN analogue with phosphodiesterase which binds to the FMN-specific apoflavodoxin. Experiments were conducted to test whether inactivation was initiated by an αHB allene carbanion or the dehydrogenation product of αHB. Kinetic studies proved inconclusive in that a rapid equilibrium between an oxidized enzyme-allene carbanion pair and reduced enzyme-keto acid pair would make these two species kinetically equivalent. The olefinic substrate 2- hydroxy-3-butenoate, however, produced no flavin adduct. Since the keto acid derived from the oxidation of this αhydroxy acid is expected to be as reactive as 2-keto-3-butynoate, it is concluded that an allene carbanion produced by abstraction of the α-hydrogen of αHB is the reactive species which covalently adds to the flavin.</dcterms:abstract> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7545"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7545/1/Suicide_inactivation_of_the_flavoenzyme_D_lactate_dehydrogenase_by_alpha_hydroxybutynoate.pdf"/> <dc:contributor>Massey, Vincent</dc:contributor> <dc:contributor>Olson, Steven T.</dc:contributor> <dc:creator>Olson, Steven T.</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Massey, Vincent</dc:creator> <dc:creator>Ghisla, Sandro</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:18Z</dc:date> <dc:language>eng</dc:language> <dcterms:title>Suicide inactivation of the flavoenzyme D-lactate dehydrogenase by alpha-hydroxybutynoate</dcterms:title> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:bibliographicCitation>First publ. in: Biochemistry 18 (1979), 21, pp. 4724- 4732</dcterms:bibliographicCitation> <dc:contributor>Whitfield, Carolyn D.</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:rights>deposit-license</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:format>application/pdf</dc:format> <dcterms:issued>1979</dcterms:issued> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Suicide_inactivation_of_the_flavoenzyme_D_lactate_dehydrogenase_by_alpha_hydroxybutynoate.pdf 136

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto