Migration of human melanoma cells depends on extracellular pH and Na+/H+ exchange


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STOCK, Christian, Birgit GASSNER, Christof R. HAUCK, Hannelore ARNOLD, Sabine MALLY, Johannes A. EBLE, Peter DIETERICH, Albrecht SCHWAB, 2005. Migration of human melanoma cells depends on extracellular pH and Na+/H+ exchange. In: The Journal of Physiology. 567(1), pp. 225-238. ISSN 0022-3751. eISSN 0022-3751

@article{Stock2005Migra-7490, title={Migration of human melanoma cells depends on extracellular pH and Na+/H+ exchange}, year={2005}, doi={10.1113/jphysiol.2005.088344}, number={1}, volume={567}, issn={0022-3751}, journal={The Journal of Physiology}, pages={225--238}, author={Stock, Christian and Gassner, Birgit and Hauck, Christof R. and Arnold, Hannelore and Mally, Sabine and Eble, Johannes A. and Dieterich, Peter and Schwab, Albrecht} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7490"> <dc:contributor>Dieterich, Peter</dc:contributor> <dc:contributor>Eble, Johannes A.</dc:contributor> <dc:creator>Mally, Sabine</dc:creator> <dc:creator>Arnold, Hannelore</dc:creator> <dc:creator>Eble, Johannes A.</dc:creator> <dcterms:issued>2005</dcterms:issued> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7490"/> <dc:contributor>Hauck, Christof R.</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:50Z</dcterms:available> <dc:creator>Schwab, Albrecht</dc:creator> <dc:format>application/pdf</dc:format> <dc:creator>Stock, Christian</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:creator>Gassner, Birgit</dc:creator> <dc:creator>Dieterich, Peter</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Schwab, Albrecht</dc:contributor> <dc:contributor>Gassner, Birgit</dc:contributor> <dc:creator>Hauck, Christof R.</dc:creator> <dc:contributor>Arnold, Hannelore</dc:contributor> <dc:contributor>Stock, Christian</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:50Z</dc:date> <dc:contributor>Mally, Sabine</dc:contributor> <dcterms:title>Migration of human melanoma cells depends on extracellular pH and Na+/H+ exchange</dcterms:title> <dcterms:bibliographicCitation>Also publ. in: The Journal of Physiology 567 (2005), 1, pp. 225 238</dcterms:bibliographicCitation> <dcterms:abstract xml:lang="eng">Their glycolytic metabolism imposes an increased acid load upon tumour cells. The surplus protons are extruded by the Na+/H+ exchanger (NHE) which causes an extracellular acidification. It is not yet known by what mechanism extracellular pH (pHe) and NHE activity affect tumour cell migration and thus metastasis. We studied the impact of pHe and NHE activity on the motility of human melanoma (MV3) cells. Cells were seeded on/in collagen I matrices. Migration was monitored employing time lapse video microscopy and then quantified as the movement of the cell centre. Intracellular pH (pHi) was measured fluorometrically. Cell matrix interactions were tested in cell adhesion assays and by the displacement of microbeads inside a collagen matrix. Migration depended on the integrin 2ß1. Cells reached their maximum motility at pHe 7.0. They hardly migrated at pHe 6.6 or 7.5, when NHE was inhibited, or when NHE activity was stimulated by loading cells with propionic acid. These procedures also caused characteristic changes in cell morphology and pHi. The changes in pHi, however, did not account for the changes in morphology and migratory behaviour. Migration and morphology more likely correlate with the strength of cell matrix interactions. Adhesion was the strongest at pHe 6.6. It weakened at basic pHe, upon NHE inhibition, or upon blockage of the integrin 2ß1. We propose that pHe and NHE activity affect migration of human melanoma cells by modulating cell matrix interactions. Migration is hindered when the interaction is too strong (acidic pHe) or too weak (alkaline pHe or NHE inhibition).</dcterms:abstract> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> </rdf:Description> </rdf:RDF>

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