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Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis : purification of sulfoacetaldehyde dehydrogenase

Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis : purification of sulfoacetaldehyde dehydrogenase

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KREJČÍK, Zdenĕk, Karin DENGER, Sonja WEINITSCHKE, Klaus HOLLEMEYER, Václav PAČES, Alasdair M. COOK, Theo H. M. SMITS, 2008. Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis : purification of sulfoacetaldehyde dehydrogenase. In: Archives of Microbiology. 190(2), pp. 159-168. ISSN 0302-8933. eISSN 1432-072X

@article{Krejcik2008Sulfo-7484, title={Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis : purification of sulfoacetaldehyde dehydrogenase}, year={2008}, doi={10.1007/s00203-008-0386-2}, number={2}, volume={190}, issn={0302-8933}, journal={Archives of Microbiology}, pages={159--168}, author={Krejčík, Zdenĕk and Denger, Karin and Weinitschke, Sonja and Hollemeyer, Klaus and Pačes, Václav and Cook, Alasdair M. and Smits, Theo H. M.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7484"> <dc:contributor>Hollemeyer, Klaus</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:48Z</dc:date> <dc:creator>Weinitschke, Sonja</dc:creator> <dc:contributor>Denger, Karin</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7484"/> <dc:language>deu</dc:language> <dc:creator>Smits, Theo H. M.</dc:creator> <dc:creator>Pačes, Václav</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:creator>Krejčík, Zdenĕk</dc:creator> <dcterms:abstract xml:lang="eng">Taurine (2-aminoethanesulfonate) is a widespread natural product whose nitrogen moiety was recently shown to be assimilated by bacteria, usually with excretion of an organosulfonate via undefined novel pathways; other data involve transcriptional regulator TauR in taurine metabolism. A screen of genome sequences for TauR with the BLAST algorithm allowed the hypothesis that the marine gammaproteobacterium Neptuniibacter caesariensis MED92 would inducibly assimilate taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter (TauABC), taurine:pyruvate aminotransferase (Tpa), a novel sulfoacetaldehyde dehydrogenase (SafD) and exporter(s) of sulfoacetate (SafE) (DUF81). Ten candidate genes in two clusters involved three sets of paralogues (for TauR, Tpa and SafE). Inducible Tpa and SafD were detected in cell extracts. SafD was purified 600-fold to homogeneity in two steps. The monomer had a molecular mass of 50 kDa (SDS-PAGE); data from gel filtration chromatography indicated a tetrameric native protein. SafD was specific for sulfoacetaldehyde with a K (m)-value of 0.12 mM. The N-terminal amino acid sequence of SafD confirmed the identity of the safD gene. The eight pathway genes were transcribed inducibly, which indicated expression of the whole hypothetical pathway. We presume that this pathway is one source of sulfoacetate in nature, where this compound is dissimilated by many bacteria.</dcterms:abstract> <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 190 (2008), 2, pp. 159-168</dcterms:bibliographicCitation> <dc:format>application/pdf</dc:format> <dc:contributor>Smits, Theo H. M.</dc:contributor> <dc:creator>Hollemeyer, Klaus</dc:creator> <dc:contributor>Weinitschke, Sonja</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:48Z</dcterms:available> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Pačes, Václav</dc:contributor> <dc:creator>Denger, Karin</dc:creator> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dcterms:issued>2008</dcterms:issued> <dc:creator>Cook, Alasdair M.</dc:creator> <dc:contributor>Krejčík, Zdenĕk</dc:contributor> <dcterms:title>Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis : purification of sulfoacetaldehyde dehydrogenase</dcterms:title> </rdf:Description> </rdf:RDF>

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